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  4. Recombinant Xenopus laevis WD repeat domain phosphoinositide-interacting protein 1 (wipi1)

Recombinant Xenopus laevis WD repeat domain phosphoinositide-interacting protein 1 (wipi1)

  • 中文名稱：
    有爪蟾蜍wipi1重組蛋白
  • 貨號：
    CSB-YP718719XBE
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍wipi1重組蛋白
  • 貨號：
    CSB-EP718719XBE
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍wipi1重組蛋白
  • 貨號：
    CSB-EP718719XBE-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍wipi1重組蛋白
  • 貨號：
    CSB-BP718719XBE
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍wipi1重組蛋白
  • 貨號：
    CSB-MP718719XBE
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    wipi1; WD repeat domain phosphoinositide-interacting protein 1; WIPI-1
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-433
  • 氨基酸序列
    METGGGGDGT GDICCLSYNQ DCTSVAIGMR SGYKLYSLSN VERLDLVHES CEAKDVYIVE RLFSSSLVVV VSHAKPRQMN VLHFKKGTEI CNYNYSDNIL SIRLNRQRLI VCLEESIYIH NIKDMKLLKT LLDTPRNPHG LCTLSINHSN SYLAYPGSSS TGEVSLYDAN CLKCECTIPA HDSPLAAIAF NSTGTKLASA SEKGTVIRVF SIPDGQKLYE FRRGMKRYVN ISSLVFSMDS QFLCASSNTE TVHVFKLEQL PERSEENASW TGYMGKMFMA ASNYLPTQVS DMMNQDRAFA TVRLNFSGQK NACTLVTIQK LPRLLVTSSS GHLYVYNLDP QDGGECVLIK KHSLLGSAKS ETDGDSDAES PVPVSYAATV ARPSSAPALS TITGYSEDGG TLRGEVIPEH ELAVGPVCLD DEKEFPPVSI KNP
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions downstream of the ulk1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists wipi2 in the recruitment of atg12-atg5-atg16l1, a complex that directly controls the elongation of the nascent autophagosomal membrane. Together with wdr45/wipi4, promotes atg2 (atg2a or atg2b)-mediated lipid transfer by enhancing atg2-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes.
  • 亞細胞定位：
    Golgi apparatus, trans-Golgi network. Endosome. Cytoplasmic vesicle, clathrin-coated vesicle. Preautophagosomal structure membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    WD repeat SVP1 family
  • 數據庫鏈接：

    KEGG: xla:446901

    UniGene: Xl.48216



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