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  4. Recombinant Xenopus laevis POU domain, class 5, transcription factor 1.1 (pou5f1.1)

Recombinant Xenopus laevis POU domain, class 5, transcription factor 1.1 (pou5f1.1)

  • 中文名稱：
    有爪蟾蜍pou5f1.1重組蛋白
  • 貨號：
    CSB-YP755987XBE
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍pou5f1.1重組蛋白
  • 貨號：
    CSB-EP755987XBE
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍pou5f1.1重組蛋白
  • 貨號：
    CSB-EP755987XBE-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍pou5f1.1重組蛋白
  • 貨號：
    CSB-BP755987XBE
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍pou5f1.1重組蛋白
  • 貨號：
    CSB-MP755987XBE
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    pou5f1.1
  • Uniprot No.：
  • 別名：
    pou5f1.1; oct-25; POU domain; class 5; transcription factor 1.1; POU class V protein oct-25; XOct-25
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-449
  • 氨基酸序列
    MYSQQPFPAF AFNAGLMQDP ANCHFGGYTG LGHPQPFSFA FSTLKSENGE SGVQGMGDCT TPVMPWNSLA SFDHQVQMEN NQQGNPPRAP SPTLSDSRIK VKEEVVHETD SGEESPEPKY PSPPNPSLYY PNAWTGAPFW QVNPTPGNNI NPMPNQTLVK NTSLPGNTTY PTPANQSPNT PVDCVTSSME SSRCSSTNSP NGAINERATT IPNGEMLDGG QSSDNEEEVP SESEMEQFAK DLKHKRVSLG YTQADVGYAL GVLYGKMFSQ TTICRFESLQ LSFKNMCQLK PFLERWVVEA ENNDNLQELI NREQVIAQTR KRKRRTNIEN IVKGTLESYF MKCPKPGAQE MVQIAKELNM DKDVVRVWFC NRRQKGKRQG MPTVEENDGE GYDVAQTMGS PPVGHYALQQ VVTPQGYMAA PQIYASAFHK NDLFPQTVPH GMAMGGHIG
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Activates transcription when directly bound to the octamer DNA sequence, but can form repression complexes with other proteins at the promoter site to inhibit transcription. Binds to the promoter of the vent2-B gene to activate transcription when in the presence of other BMP signaling factors also bound to the promoter. Inhibits the competence of ectodermal cells to respond to BMP during embryogenesis thereby inhibiting epidermal differentiation and promoting neural induction. Antagonizes the activity of nodal/activin signaling by forming a transcriptional repression complex on the gsc and mix2 gene promoters to inhibit their transcription, and thus maintain the undifferentiated state of embryonic cells to prevent them from differentiating prematurely. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription by forming a transcriptional repression complex on the nodal/nr1 and siamois promoters to inhibit their transcription.
  • 基因功能參考文獻：
    1. results suggest that one role of pou5f3.2 is to keep the embryonic cells in undifferentiated and proliferative state during gastrulation PMID: 26660397
    2. A conserved Oct4-dependent network links cell adhesion and migration to progenitor cell maintenance. PMID: 24210613
    3. These results suggest that Sall4, activated by posteriorizing signals, represses the pou5f3 genes to provide a permissive environment. PMID: 24715458
    4. we conclude that the epistatic relationship between Suv4-20h enzymes and Oct-25 controls the transit from pluripotent to differentiation-competent neural cells PMID: 23382689
    5. Oct25 affects morphogenesis via activation of PAPC expression and subsequent functional inhibition of EP/C-cadherin. PMID: 22957893
    6. nuclear actin polymerization plays an essential part in the transcriptional reactivation of the pluripotency gene Oct4 (also known as Pou5f1). PMID: 21536734
    7. Oct-25 stimulates transcription of the Xvent-2B gene. PMID: 15292233
    8. the integrity of the POU domain is crucial for the function of POU-V transcription factors in the regulation of genes that promote germ layer formation PMID: 20064932
    9. These results document an essential role for XOct-25 in commitment to neural or epidermal cell fates in the ectoderm. PMID: 17950579
    10. Oct25 controls the nodal/activin pathway and thus maintains the undifferentiated state of embryonic cells in preventing them from premature differentiation PMID: 18922797

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    POU transcription factor family, Class-5 subfamily
  • 組織特異性：
    Highly enriched within the animal half of developing embryos within ectodermal and mesodermal regions. Expressed in the neuroectoderm at the early neurula stage, with expression initially extending to the future hindbrain/midbrain boundary, but later shif
  • 數(shù)據(jù)庫鏈接：

    KEGG: xla:379522

    UniGene: Xl.4957



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