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  4. Recombinant Xenopus laevis Nuclear cap-binding protein subunit 2 (ncbp2)

Recombinant Xenopus laevis Nuclear cap-binding protein subunit 2 (ncbp2)

  • 中文名稱：
    有爪蟾蜍ncbp2重組蛋白
  • 貨號：
    CSB-YP015521XBE
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍ncbp2重組蛋白
  • 貨號：
    CSB-EP015521XBE
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍ncbp2重組蛋白
  • 貨號：
    CSB-EP015521XBE-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍ncbp2重組蛋白
  • 貨號：
    CSB-BP015521XBE
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍ncbp2重組蛋白
  • 貨號：
    CSB-MP015521XBE
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    ncbp2; cbp20Nuclear cap-binding protein subunit 2; 20 kDa nuclear cap-binding protein; NCBP 20 kDa subunit; CBP20
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-153
  • 氨基酸序列
    MALSALRSDS YVDLSQYRDQ HFRGNRSDQE SLLKQSCTLY VGNLSFYTTE EQIHELFSKS GDVKRIVMGL DKVKKTACGF CFVEYYTRAD AEQAMRFING TRLDDRIVRT DWDAGFKEGR QYGRGKSGGQ VRDEYRQDYD AGRGGYGKVV QSF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC), promoting the interaction between upf1 and upf2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with srrt/ars2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of parn, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, ncbp2/cbp20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires ncbp1/cbp80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus.
  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    RRM NCBP2 family
  • 數據庫鏈接：

    KEGG: xla:100380998

    UniGene: Xl.43439



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