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  4. Recombinant Xenopus laevis Noggin (nog)

Recombinant Xenopus laevis Noggin (nog)

  • 中文名稱：
    有爪蟾蜍nog重組蛋白
  • 貨號：
    CSB-YP015917XBE
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍nog重組蛋白
  • 貨號：
    CSB-EP015917XBE
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍nog重組蛋白
  • 貨號：
    CSB-EP015917XBE-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍nog重組蛋白
  • 貨號：
    CSB-BP015917XBE
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍nog重組蛋白
  • 貨號：
    CSB-MP015917XBE
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    nog; Noggin
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    20-222
  • 氨基酸序列
    R IDQGGCQHYL HIRPAPSENL PLVDLIEHPD PIYDPKEKDL NETLLRTLMV GHFDPNFMAT ILPEERLGVE DLGELDLLLR QKPSGAMPAE IKGLEFYEGL QSKKHRLSKK LRRKLQMWLW SQTFCPVLYT WNDLGTRFWP RYVKVGSCYS KRSCSVPEGM VCKAAKSMHL TILRWRCQRR VQQKCAWITI QYPVISECKC SC
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Patterns the embryo by interrupting bone morphogenetic proteins (BMP) signaling. Binds BMP-4 and BMP-2 with high affinity. Can abolish BMP-4 activity by blocking binding to cognate cell-surface receptors. Capable of inducing dorsal development in embryos. Causes dorsal mesodermal differentiation of animal cap ectoderm when coexpressed with xWNT-8 and nuclear, sequence-specific DNA-binding protein xBRA. None of these molecules causes dorsal mesoderm formation when expressed alone.
  • 基因功能參考文獻：
    1. noggin 1, differently from noggin 2 and noggin 4, is expressed during all phases of Xenopus laevis retinal development. Gain-of-function experiments by electroporation in the optic vesicle show that overexpression of noggin 1 significantly decreases the number of bipolar cells in the inner nuclear layer of the retina, without significantly affecting the generation of the other retinal cell types. PMID: 27389985
    2. a new role for Noggin1 in determining specific anterior neural structures by the modulation of TGFbeta and SHH signaling. PMID: 25913744
    3. The data provide a quantitative basis for modeling the process of Noggin1 diffusion in embryonic tissues, considering its interaction with heparan sulfate proteoglycans. PMID: 26525852
    4. This allow one to compare the expression levels of Noggin1 and Noggin 2 constructs, to purify them on the affine immunosorbent and to show the activity of Noggin proteins by analyzing their ability to bind BMP4 factor PMID: 23964527
    5. Both Noggin proteins could induce a secondary head, including the forebrain. During normal development, Noggin1 mRNA was translated with low efficiency, providing sufficient Noggin1 only for antagonizing Bone morphogenetic protein. PMID: 22071106
    6. A 2066 bp noggin 5' flanking sequence which recapitulates the roof-plate expression of endogenous gene in transgenic frog tadpoles has been identified; this roof-plate enhancer has been mapped to a sequence as short as 79 bp. PMID: 14625440
    7. Noggin specifically blocks chondrogenic differentiation, rather than osteogenic differentiation, in mesodermal stem cell line C1 and skeletal cells. PMID: 15044373
    8. Chordin, Noggin, beta-Catenin, and Cerberus have roles in neural induction in Xenopus PMID: 15138495
    9. X-epilectin expression is down-regulated by Noggin and tBR and that this effect is inhibited by BMP4 over-expression, suggesting X-epilectin expression is mediated by the BMP signalling pathway PMID: 15602698
    10. maternal mRNA encoding noggin is enriched in animal tiers and at low concentrations in the C-tier, suggesting that the neural fates of C-tier blastomeres may be responsive to early signaling from their neighboring cells PMID: 17096409

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Noggin family
  • 數據庫鏈接：

    KEGG: xla:373646

    UniGene: Xl.834



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