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  4. Recombinant Xenopus laevis Frizzled-7-A (fzd7-a), partial

Recombinant Xenopus laevis Frizzled-7-A (fzd7-a), partial

  • 中文名稱：
    有爪蟾蜍fzd7-a重組蛋白
  • 貨號：
    CSB-YP882486XBE1
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍fzd7-a重組蛋白
  • 貨號：
    CSB-EP882486XBE1
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍fzd7-a重組蛋白
  • 貨號：
    CSB-EP882486XBE1-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍fzd7-a重組蛋白
  • 貨號：
    CSB-BP882486XBE1
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍fzd7-a重組蛋白
  • 貨號：
    CSB-MP882486XBE1
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    fzd7-a
  • Uniprot No.：
  • 別名：
    fzd7-a; fz7-a; Frizzled-7-A; Fz-7-A; Xfz7-A
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Receptor for Wnt proteins. Acts in both canonical and non-canonical Wnt pathways. Although different papers report differing Wnt preferences, wnt5a, wnt8b and wnt11 have been proposed as synergists. In the canonical Wnt pathway, acts via beta-catenin to promote the expression of the dorsal genes siamois, twin and nodal3 and to establish the dorsal axis of the embryo and induce dorsal mesoderm formation. In a non-canonical Wnt/planar cell polarity (PCP) pathway, acts with sdc4 and dvl2/dsh to regulate convergent extension cell movements during gastrulation. Triggers phosphorylation of dvl2/dsh and its translocation to the plasma membrane. In a third branch of Wnt signaling, acts in a non-canonical pathway via trimeric G proteins, and independently of dvl2/dsh, to recruit protein kinase C (PKC) to the membrane and thus activate PKC. PKC signaling controls cell sorting and tissue separation during gastrulation.
  • 基因功能參考文獻：
    1. in the foregut Fzd7 signals via both the Wnt/beta-catenin and Wnt/JNK pathways and different thresholds of Wnt11, 5 and 8-Fzd7 activity coordinate progenitor cell fate, proliferation and morphogenesis PMID: 23562607
    2. Because paraxial protocadherin and C-cadherin do not directly interact nor form a joint complex with Fz7, Wnt-11 triggers the formation of two distinct complexes that act in parallel to reduce cell adhesion by hampering clustering of C-cadherin. PMID: 22908314
    3. We therefore demonstrate that Xfz7 is regulated by BMP antagonism and is required for neural crest induction by Wnt in the developing vertebrate embryo. PMID: 16928367
    4. Wnt11-induced polarized accumulation of Frizzled7 and Dishevelled at adherens junctions underlies the formation and maintenance of apicobasal cell polarity. PMID: 17663724
    5. Results suggest that Ryk functions as an essential regulator for noncanonical Wnt11/frizzled 7-mediated endocytosis in the regulation of Xenopus laevis convergent extension movements. PMID: 18809723

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor Fz/Smo family
  • 組織特異性：
    Expressed in the animal region of cleavage stage embryos. During gastrulation, broadly expressed on the dorsal side of the embryo in deep mesodermal cells surrounding the blastopore lip and in presumptive anterior neuroectoderm. During neurulation, become
  • 數據庫鏈接：

    KEGG: xla:378787

    UniGene: Xl.633



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