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  4. Recombinant Xenopus laevis Eukaryotic initiation factor 4A-III-B (eif4a3-b)

Recombinant Xenopus laevis Eukaryotic initiation factor 4A-III-B (eif4a3-b)

  • 中文名稱：
    有爪蟾蜍eif4a3-b重組蛋白
  • 貨號：
    CSB-YP522095XBE
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍eif4a3-b重組蛋白
  • 貨號：
    CSB-EP522095XBE
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍eif4a3-b重組蛋白
  • 貨號：
    CSB-EP522095XBE-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍eif4a3-b重組蛋白
  • 貨號：
    CSB-BP522095XBE
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍eif4a3-b重組蛋白
  • 貨號：
    CSB-MP522095XBE
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    eif4a3-b
  • Uniprot No.：
  • 別名：
    eif4a3-b; ddx48Eukaryotic initiation factor 4A-III-B; XeIF-4AIII; eIF-4A-III-B; eIF4A-III-B; EC 3.6.4.13; ATP-dependent RNA helicase DDX48-B; ATP-dependent RNA helicase eIF4A-3-B; DEAD box protein 48-B; Eukaryotic translation initiation factor 4A isoform 3-B
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-414
  • 氨基酸序列
    MAAAAVAGVA GLTTAHAKRL LREEDMTTVE FQTSEEVDVT PTFDTMGLRE DLLRGIYAYG FEKPSAIQQK AIKQIIKGRD VIAQSQSGTG KTATFCVSVL QCLDIQIRET QALILAPTKE LARQIQKVLL ALGDYMNVQC HACIGGTNVG EDIRKLDYGQ HVVAGTPGRV FDMIRRRSLR TRAIKMLVLD EADEMLNKGF KEQIYDVYRY LPPATQVCLI SATLPHEILE MTNKFMTDPI RILVKRDELT LEGIKQFFVA VEREEWKFDT LCDLYDTLTI TQAVIFCNTK RKVDWLTEKM REANFTVSSM HGDMPQKERE SIMKEFRSGA SRVLISTDVW ARGLDVPQVS LIINYDLPNN RELYIHRIGR SGRYGRKGVA INFVKNDDIR ILRDIEQYYS TQIDEMPMNV ADLI
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Involved in craniofacial development. When overexpressed, induces epidermis in dissociated cells that would otherwise adopt a neural fate, a process that requires an active BMP signaling pathway.
  • 基因功能參考文獻：
    1. Eif4a3 is required for accurate splicing of the Xenopus laevis ryanodine receptor pre-mRNA PMID: 22944195
    2. Knockdown of the EJC component Eukaryotic initiation factor 4a3 (Eif4a3) in embryos of the frog Xenopus laevis results in full-body paralysis, with defects in sensory neuron, pigment cell, and cardiac development. PMID: 20549732
  • 亞細胞定位：
    Nucleus. Nucleus speckle. Cytoplasm.
  • 蛋白家族：
    DEAD box helicase family
  • 數據庫鏈接：

    KEGG: xla:399362

    UniGene: Xl.755



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