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  4. Recombinant Xenopus laevis Aurora kinase A-A (aurka-a)

Recombinant Xenopus laevis Aurora kinase A-A (aurka-a)

  • 中文名稱：
    Recombinant Xenopus laevis Aurora kinase A-A(aurka-a)
  • 貨號(hào)：
    CSB-YP838633XBE
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Xenopus laevis Aurora kinase A-A(aurka-a)
  • 貨號(hào)：
    CSB-EP838633XBE
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Xenopus laevis Aurora kinase A-A(aurka-a)
  • 貨號(hào)：
    CSB-EP838633XBE-B
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Xenopus laevis Aurora kinase A-A(aurka-a)
  • 貨號(hào)：
    CSB-BP838633XBE
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Xenopus laevis Aurora kinase A-A(aurka-a)
  • 貨號(hào)：
    CSB-MP838633XBE
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    aurka-a
  • Uniprot No.：
  • 別名：
    aurka-a; aurka; eg2; stk6Aurora kinase A-A; EC 2.7.11.1; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; Serine/threonine-protein kinase 6-A; Serine/threonine-protein kinase Eg2-A; pEg2; Serine/threonine-protein kinase aurora-A; p46Eg265
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-407
  • 氨基酸序列
    MERAVKENHK PSNVKIFHPM TEGAKRIPVN QPQSTQFRPP GTAVSAQRIL GPSNVPQRVL AQAQKPILSS QKPTTQIPLR PATQGHQSSK PQGPNENRNP QQTSHSSTPN VEKKGSTDQG KTSAVPKEEG KKKQWCLEDF EIGRPLGKGK FGNVYLARER ESKFILALKV LFKSQLEKAG VEHQLRREVE IQSHLRHPNI LRLYGYFHDA SRVYLILDYA PGGELFRELQ KCTRFDDQRS AMYIKQLAEA LLYCHSKKVI HRDIKPENLL LGSNGELKIA DFGWSVHAPS SRRTTLCGTL DYLPPEMIEG RMHDETVDLW SLGVLCYEFL VGKPPFETDT HQETYRRISK VEFQYPPYVS EEARDLVSKL LKHNPNHRLP LKGVLEHPWI IKNSQLKKKD EPLPGAQ
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Phosphorylates numerous target proteins. Important for microtubule formation and/or stabilization.
  • 基因功能參考文獻(xiàn)：
    1. These results suggest a novel relationship between AurA and protein phosphatases during progression throughout the early embryonic cell cycle and shed new light on potential defects caused by AurA overexpression. PMID: 24825897
    2. Aurora-A kinase is required for astral microtubule polymerization and spindle microtubule flux during chromosome segregation. PMID: 18414060
    3. Data show that Aurora A is a key regulator of microtubule assembly during M phase and therefore of bipolar spindle formation. PMID: 18756265
    4. binding and elution properties of both the phosphopeptides and unphosphorylated peptides of His6-Aurora A PMID: 12885952
    5. Results suggest that phosphorylation of maskin by Aurora-A prevents meiosis II proteins from being produced during meiosis I. PMID: 15687499
    6. The N-terminal non-catalytic domain of Aurora-A can localize to the centrosome in Xenopus egg extracts, while GFP fusions of either the N-terminal or catalytic domains are targeted to the centrosome in Xenopus XL2 cells. PMID: 15182704
    7. Here we identify G205 in Xenopus Aurora A as a key determinant of both intrinsic activity and regulation by TPX2 PMID: 15908779
    8. The catalytic domain alone of Aurora-A is sufficient to restore spindle bipolarity; additional N-terminal sequences function in mitotic timing. PMID: 16581905

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  • 亞細(xì)胞定位：
    Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily
  • 組織特異性：
    Highly expressed in ovary and testis.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: xla:397925

    UniGene: Xl.124



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