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  4. Recombinant Vaccinia virus mRNA-capping enzyme catalytic subunit (VACWR106), partial

Recombinant Vaccinia virus mRNA-capping enzyme catalytic subunit (VACWR106), partial

  • 中文名稱：
    Recombinant Vaccinia virus mRNA-capping enzyme catalytic subunit (VACWR106) ,partial
  • 貨號：
    CSB-YP366132VAI
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus mRNA-capping enzyme catalytic subunit (VACWR106) ,partial
  • 貨號：
    CSB-EP366132VAI
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus mRNA-capping enzyme catalytic subunit (VACWR106) ,partial
  • 貨號：
    CSB-EP366132VAI-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus mRNA-capping enzyme catalytic subunit (VACWR106) ,partial
  • 貨號：
    CSB-BP366132VAI
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus mRNA-capping enzyme catalytic subunit (VACWR106) ,partial
  • 貨號：
    CSB-MP366132VAI
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    VACWR106
  • Uniprot No.：
  • 別名：
    VACWR106; D1RmRNA-capping enzyme catalytic subunit; Virus termination factor large subunit; VTF large subunit; mRNA-capping enzyme 97 kDa subunit; mRNA-capping enzyme D1 subunit; mRNA-capping enzyme large subunit) [Includes: Polynucleotide 5'-triphosphatase; EC 3.1.3.33; mRNA 5'-triphosphatase; TPase); mRNA guanylyltransferase; EC 2.7.7.50; GTP--RNA guanylyltransferase; GTase); mRNA; guanine-N(7)-)-methyltransferase; EC 2.1.1.56; mRNA cap methyltransferase)]
  • 種屬：
    Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalytic subunit of the mRNA capping enzyme which catalyzes three enzymatic reactions: the 5' triphosphate end of the pre-mRNA is hydrolyzed to a diphosphate by RNA 5' triphosphatase; the diphosphate RNA end is capped with GMP by RNA guanylyltransferase and the GpppN cap is methylated by RNA (guanine-N7) methyltransferase. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the polymerase associated VP39 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure).; The heterodimeric enzyme is also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I and the Rap94 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA.
  • 基因功能參考文獻：
    1. VTF provides the connection between the termination signal in the RNA transcript and viral RNA polymerase to initiate transcription termination. PMID: 26280468
    2. Crystal structure of vaccinia virus mRNA capping enzyme PMID: 24607143
    3. the results presented in this study provide the first in vivo evidence that the vaccinia virus mRNA capping enzyme plays a role in early gene transcription termination and intermediate gene transcription. PMID: 18295814
  • 亞細胞定位：
    Virion. Note=All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome.
  • 蛋白家族：
    DsDNA virus mRNA guanylyltransferase family; Class I-like SAM-binding methyltransferase superfamily, mRNA cap 0 methyltransferase family
  • 數據庫鏈接：

    KEGG: vg:3707562



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