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  4. Recombinant Vaccinia virus Serine/threonine-protein kinase 1 (VPK1)

Recombinant Vaccinia virus Serine/threonine-protein kinase 1 (VPK1)

  • 中文名稱：
    Recombinant Vaccinia virus Serine/threonine-protein kinase 1(VPK1)
  • 貨號：
    CSB-YP527560VEE
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus Serine/threonine-protein kinase 1(VPK1)
  • 貨號：
    CSB-EP527560VEE
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus Serine/threonine-protein kinase 1(VPK1)
  • 貨號：
    CSB-EP527560VEE-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus Serine/threonine-protein kinase 1(VPK1)
  • 貨號：
    CSB-BP527560VEE
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus Serine/threonine-protein kinase 1(VPK1)
  • 貨號：
    CSB-MP527560VEE
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    VPK1
  • Uniprot No.：
  • 別名：
    VPK1; MVA167R; ACAM3000_MVA_167; B1R; B1 kinase; Serine/threonine-protein kinase 1; EC 2.7.11.1
  • 種屬：
    Vaccinia virus (strain Ankara) (VACV)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-300
  • 氨基酸序列
    MNFQGLVLTD NCKNQWVVGP LIGKGGFGSI YTTNDNNYVV KIEPKANGSL FTEQAFYTRV LKPSVIEEWK KSHNIKHVGL ITCKAFGLYK SINVEYRFLV INRLGADLDA VIRANNNRLP KRSVMLIGIE ILNTIQFMHE QGYSHGDIKA SNIVLDQIDK NKLYLVDYGL VSKFMSNGEH VPFIRNPNKM DNGTLEFTPI DSHKGYVVSR RGDLETLGYC MIRWLGGILP WTKISETKNC ALVSATKQKY VNNTATLLMT SLQYAPRELL QYITMVNSLT YFEEPNYDKF RHILMQGVYY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Essential serine/threonine-protein kinase that plays different role in the viral life cycle. Phosphorylates the host small ribosomal protein RACK1 thereby customizing the ribosomes to a state optimal for viral mRNAs (which contain poly-A leaders) but not for host mRNAs. Facilitates viral DNA replication by inhibiting host BANF1, a cellular host defense responsive to foreign DNA. Phosphorylates host BANF1 on serine and threonine residues; this leads to BANF1 relocalization to the cytoplasm, loss of dimerization and impaired DNA binding activity. Indeed, BANF1 activity depends on its DNA-binding property which is blocked by VPK1-mediated phosphorylation. Required for viral intermediate genes expression, probably by inhibiting host BANF1. Modulates cellular responses via host JUN by two different mechanisms, either by direct phosphorylation or by modulation of upstream JIP1-MAPK complexes. Seems to participate in the accumulation/processing of late proteins and thus in virion maturation.
  • 亞細胞定位：
    Virion. Host cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, Poxviruses subfamily


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