Recombinant Triticum aestivum Omega-5 gliadin, partial
In Stock-
中文名稱:小麥Omega-5麥谷蛋白重組蛋白
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貨號:CSB-EP2020TQN
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規格:¥2328
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圖片:
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其他:
產品詳情
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純度:Greater than 90% as determined by SDS-PAGE.
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Uniprot No.:
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種屬:Triticum aestivum (Wheat)
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蛋白長度:Partial
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來源:E.coli
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分子量:37.5kDa
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表達區域:262-439aa
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氨基酸序列QQQFPQQQSPQQQQFPQQQFPQQQQLPQKQFPQPQQIPQQQQIPQQPQQFPQQQFPQQQQFPQQQEFPQQQFPQQQFHQQQLPQQQFPQQQFPQQQFPQQQQFPQQQQLTQQQFPRPQQSPEQQQFPQQQFPQQPPQQFPQQQFPIPYPPQQSEEPSPYQQYPQQQPSGSDVISISGL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering. -
蛋白標簽:N-terminal 6xHis-SUMO-tagged
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產品提供形式:Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. -
儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:3-7 business days
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注意事項:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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產品描述:
Recombinant Triticum aestivum Omega-5 gliadin is an E.coli?cell-expressed partial?protein. E.coli?expression systems are popular because bacteria are easy to culture, grow fast and produce high yields of recombinant protein. Traditional strategies for Triticum aestivum Omega-5 gliadin recombinant protein expression involve transforming the recombinant plasmid into the E. coli host. The cells were directly replicated, transcribed and translated, and IPTG was added to express the desired protein. Typically, the cells are then lysed to extract the expressed protein for subsequent purification. Also, many proteins become insoluble as inclusion bodies that are very difficult to recover, so mild conditions and subsequent cumbersome protein-refolding procedures are used to ensure the final provided protein is soluble.
Omega-5 gliadin is a protein component of gluten in triticum aestivum (wheat). Different to gliten proteins, omega gliadins are distinguished from the others on the basis of their amino acid compositions, being exceptionally rich in proline and glutamine with little or no methionine and cysteine. TOf the wheat allergens, omega-5 gliadin has been implicated as the most frequent trigger of WDEIA. Omega-5 gliadin allergy is a rare wheat allergy that presents with severe anaphylaxis. Exercise is the key co-factor, and other known co-factors include alcohol, menstruation and aspirin/NSAIDs. -
Datasheet & COA:Please contact us to get it.
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