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  4. Recombinant Tomato yellow leaf curl Sardinia virus Replication-associated protein (C1)

Recombinant Tomato yellow leaf curl Sardinia virus Replication-associated protein (C1)

  • 中文名稱：
    Recombinant Tomato yellow leaf curl Sardinia virus Replication-associated protein (C1)
  • 貨號：
    CSB-YP340861TGT
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Tomato yellow leaf curl Sardinia virus Replication-associated protein (C1)
  • 貨號：
    CSB-EP340861TGT
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Tomato yellow leaf curl Sardinia virus Replication-associated protein (C1)
  • 貨號：
    CSB-EP340861TGT-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Tomato yellow leaf curl Sardinia virus Replication-associated protein (C1)
  • 貨號：
    CSB-BP340861TGT
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Tomato yellow leaf curl Sardinia virus Replication-associated protein (C1)
  • 貨號：
    CSB-MP340861TGT
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    C1
  • Uniprot No.：
  • 別名：
    C1; Replication-associated protein; Rep; EC 2.7.7.-; EC 3.1.21.-; Protein C1
  • 種屬：
    Tomato yellow leaf curl Sardinia virus (TYLCSV)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-359
  • 氨基酸序列
    MPRSGRFSIK AKNYFLTYPK CDLTKENALS QITNLQTPTN KLFIKICREL HENGEPHLHI LIQFEGKYNC TNQRFFDLVS PTRSAHFHPN IQGAKSSSDV KSYIDKDGDV LEWGTFQIDG RSARGGQQTA NDAYAKAINA GSKSQALDVI KELAPRDYVL HFHNINSNLD KVFQVPPAPY VSPFLSSSFD QVPDELEHWV SENVMDAAAR PWRPVSIVIE GDSRTGKTTW ARSLGPHNYL CGHLDLSQKV YSNNAWYNVI DDVDPHYLKH FKEFMGAQRD WQSNTKYGKP IQIKGGIPTI FLCNPGPQSS FKEYLDEEKN QALKNWATKN AIFVTIHQPL FADTNQNTTS HRQEEASEA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.
  • 基因功能參考文獻：
    1. The RGG sequence in Rep appears relevant for the transcriptional control of the C1-gene and for the inhibition of viral replication and dramatically impacts resistance in transgenic plants. PMID: 20943892
    2. results show that Rep lacking the 121 N-terminal amino acids has helicase activity comparable to that of the other helicases: requirements for a 3' overhang & 3'-to-5' polarity of unwinding, with some distinct features & with a minimal AAA+ ATPase domain PMID: 16943286
  • 亞細胞定位：
    Host nucleus.
  • 蛋白家族：
    Geminiviridae Rep protein family
  • 數據庫鏈接：

    KEGG: vg:944425



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