Recombinant Thymidylate synthase (thyA)

中文名稱：

大腸桿菌O157：H7thyA重組蛋白
貨號(hào)：

CSB-YP025393EOD
規(guī)格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌O157：H7thyA重組蛋白
貨號(hào)：

CSB-EP025393EOD
規(guī)格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌O157：H7thyA重組蛋白
貨號(hào)：

CSB-EP025393EOD-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌O157：H7thyA重組蛋白
貨號(hào)：

CSB-BP025393EOD
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌O157：H7thyA重組蛋白
貨號(hào)：

CSB-MP025393EOD
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

thyA
Uniprot No.：

P0A886
別名：

thyA; Z4144; ECs3684; Thymidylate synthase; TS; TSase; EC 2.1.1.45
種屬：

Escherichia coli O157:H7
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-264
氨基酸序列

MKQYLELMQK VLDEGTQKND RTGTGTLSIF GHQMRFNLQD GFPLVTTKRC HLRSIIHELL WFLQGDTNIA YLHENNVTIW DEWADENGDL GPVYGKQWRA WPTPDGRHID QITTVLNQLK NDPDSRRIIV SAWNVGELDK MALAPCHAFF QFYVADGKLS CQLYQRSCDV FLGLPFNIAS YALLVHMMAQ QCDLEVGDFV WTGGDTHLYS NHMDQTHLQL SREPRPLPKL IIKRKPESIF DYRFEDFEIE GYDPHPGIKA PVAI
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.
基因功能參考文獻(xiàn)：
1. We report the 2.4 A X-ray structure of the C-terminal deletion mutant ofEscherichia coli thymidylate synthase in complex with a substrate and a cofactor analogue, CB3717. PMID: 29717875
亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Thymidylate synthase family, Bacterial-type ThyA subfamily
數(shù)據(jù)庫鏈接：

KEGG: ece:Z4144

STRING: 155864.Z4144