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  4. Recombinant Tetrahymena thermophila Histone H3.3 (HHT3)

Recombinant Tetrahymena thermophila Histone H3.3 (HHT3)

  • 中文名稱：
    Recombinant Tetrahymena thermophila Histone H3.3(HHT3)
  • 貨號(hào)：
    CSB-YP010109TKA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Tetrahymena thermophila Histone H3.3(HHT3)
  • 貨號(hào)：
    CSB-EP010109TKA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Tetrahymena thermophila Histone H3.3(HHT3)
  • 貨號(hào)：
    CSB-EP010109TKA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Tetrahymena thermophila Histone H3.3(HHT3)
  • 貨號(hào)：
    CSB-BP010109TKA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Tetrahymena thermophila Histone H3.3(HHT3)
  • 貨號(hào)：
    CSB-MP010109TKA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    HHT3
  • Uniprot No.：
  • 別名：
    HHT3; TTHERM_00016170; Histone H3.3; Minor histone H3 variant; hv2
  • 種屬：
    Tetrahymena thermophila (strain SB210)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    2-136
  • 氨基酸序列
    ARTKQTARK STGVKAPRKQ LATKAARKSA PVSGGVKKPH KFRPGTVALR EIRKYQKTTD LLIRKLPFQR LVRDIAMEMK SDIRFQSQAI LALQEAAEAY LVGLFEDTNL CAIHARRVTI MTKDLHLARR IRGERF
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Macronuclear replacement variant which replaces conventional H3 in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Functions redundantly to H3.4. H3.3 deposition into chromatin is mainly transcription-associated and DNA replication-independent, but it can also enter a replication-coupled pathway. Although not essential for vegetative growth, minor H3 variants are required for producing viable conjugation progeny by affecting late developmental stages of conjugation.
  • 基因功能參考文獻(xiàn)：
    1. Global changes in histone post-translational modifications in DeltaTXR1 and DeltaEZL2 cells were quantified, to systematically identify potential crosstalk between H3K27 methylation and other posttranslational modifications. PMID: 24382802
  • 亞細(xì)胞定位：
    Nucleus. Chromosome. Note=Localizes mainly to the large, transcriptionally active, somatic macronucleus (MAC) and only faintly to the small, transcriptionally inert, germ line micronucleus (MIC).
  • 蛋白家族：
    Histone H3 family
  • 數(shù)據(jù)庫(kù)鏈接：


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