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  4. Recombinant Systemic RNA interference defective protein 1 (sid-1), partial

Recombinant Systemic RNA interference defective protein 1 (sid-1), partial

  • 中文名稱：
    秀麗隱桿線蟲sid-1重組蛋白
  • 貨號：
    CSB-YP880921CXY
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲sid-1重組蛋白
  • 貨號：
    CSB-EP880921CXY
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲sid-1重組蛋白
  • 貨號：
    CSB-EP880921CXY-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲sid-1重組蛋白
  • 貨號：
    CSB-BP880921CXY
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲sid-1重組蛋白
  • 貨號：
    CSB-MP880921CXY
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    sid-1
  • Uniprot No.：
  • 別名：
    sid-1; C04F5.1; Systemic RNA interference defective protein 1; Systemic RNAi enabling protein
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Plays a role in RNA-mediated gene silencing by acting cell-autonomously as a channel for the transport of double-stranded RNA (dsRNA) between cells. Mediates the spread of dsRNA and subsequent silencing of genes in cells distant from the site of dsRNA introduction. Selective for dsRNA. Preferentially binds long dsRNA, from 50 base pairs up to 700. Short 20 base-pair long molecules are not bound. May also bind dsDNA, but with lower affinity. Binding may be sequence-independent. Required for avoidance behavior induced by small RNAs derived from pathogenic bacteria such as P.aeruginosa.
  • 基因功能參考文獻：
    1. consistent with previous studies, we fail to detect a requirement for sid-1 in dsRNA export, but demonstrate for the first time that SID-1 functions in the intestine to support environmental RNAi (eRNAi). PMID: 29025917
    2. sid-1 is required for inheritance of silencing RNAs. dsRNA transit through the endocytosis pathway in early embryos is independent of sid-1. PMID: 28751423
    3. Epigenetic silencing of the endogenous sid-1 locus is maintained by germline RNAi-mediated inheritance for up to 13 generations. PMID: 28212751
    4. The functional importance of SID-1 extracellular domain as a dsRNA binding domain that contributes to RNA transport. PMID: 26067272
    5. SID-1 is a dsRNA-selective dsRNA-gated channel. PMID: 21474576
    6. C. elegans expressing neuronal SID-1 showed RNAi phenotypes when fed with bacteria expressing dsRNA for known neuronal genes and for uncharacterized genes with no previously known neuronal phenotypes. PMID: 20512143
    7. data indicate that systemic RNA interference in C. elegans involves SID-1-mediated intercellular transport of dsRNA PMID: 12970568
    8. Study shows that equivalent masses of long or short dsRNA accumulate in Drosophila S2 cells, indicating that size-dependent silencing is not due to size-selective transport through SID-1. PMID: 19155320
    9. C. elegans can transport endogenous and exogenous RNA silencing signals between many different tissues via at least 2 SID-1 independent export pathways PMID: 19168628

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein. Note=Enriched at the cell periphery and also to the punctate structures of cytoplasm.
  • 蛋白家族：
    SID1 family
  • 組織特異性：
    Expressed in most non-neuronal cells, including body wall muscle cells.
  • 數據庫鏈接：

    KEGG: cel:CELE_C04F5.1

    STRING: 6239.C04F5.1

    UniGene: Cel.179



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