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  4. Recombinant Synembryn (ric-8), partial

Recombinant Synembryn (ric-8), partial

  • 中文名稱：
    秀麗隱桿線蟲ric-8重組蛋白
  • 貨號：
    CSB-YP880916CXY
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ric-8重組蛋白
  • 貨號：
    CSB-EP880916CXY
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ric-8重組蛋白
  • 貨號：
    CSB-EP880916CXY-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ric-8重組蛋白
  • 貨號：
    CSB-BP880916CXY
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ric-8重組蛋白
  • 貨號：
    CSB-MP880916CXY
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ric-8
  • Uniprot No.：
  • 別名：
    ric-8; Y69A2AR.2; Synembryn; Resistance to inhibitors of cholinesterase protein 8
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins independently of G-protein coupled receptors. Acts by exchanging bound GDP for free GTP. Able to facilitate synaptic transmission in the nervous system probably by activating G(q)-alpha (egl-30). Also able to activate the G(s)-alpha in synaptic signaling network. Plays a key role in asymmetric spindle positioning, a step for asymmetric cell division that generates cell diversity during development by activating G(i)-alpha protein goa-1 and gpa-16 independently of G-protein coupled receptors. While it acts as a GEF for goa-1, it has no GEF activity toward gpa-16. In addition to its GEF activity, it is required for cortical subcellular localization of G-alpha proteins such as gpa-16. Also required for the interaction of goa-1 and gpr-1/2, suggesting that it may act by generating G-alpha proteins free from G-beta-gamma subunits, enabling gpr-1/2 to mediate asymmetric cell division.
  • 基因功能參考文獻：
    1. Genetic epistasis experiments show that activator of G protein signaling (AGS-3) and guanine nucleotide exchange factor RIC-8 act during food deprivation in a mutually dependent fashion to activate G protein Galpha(o). PMID: 21832186
    2. ric-8 (synembryn) paralysis can be rescued by neuronal G alpha(s) pathway activation. PMID: 15489510
    3. Analysis of a ric-8 mutant suggests that it is required to maintain both the G alpha(q) vesicle priming pathway and the neuronal G alpha(s) pathway in a functional state. PMID: 15489511
    4. RIC-8 directly modulates Galpha activity and that Galpha-GTP is the signaling molecule regulating spindle positioning in the early embryo PMID: 15498497
  • 亞細胞定位：
    Cytoplasm, cell cortex. Note=Localizes to the cell cortex. Excluded from interphase nuclei and becomes localized around what appeared to be kinetochore microtubules at the onset of mitosis, becoming more diffuse during anaphase.
  • 蛋白家族：
    Synembryn family
  • 組織特異性：
    Present throughout the nervous system in juveniles and adults (at protein level).
  • 數據庫鏈接：


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