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  4. Recombinant Synechocystis sp. Ribonuclease E homolog (rne), partial

Recombinant Synechocystis sp. Ribonuclease E homolog (rne), partial

  • 中文名稱：
    聚囊藻屬rne重組蛋白
  • 貨號：
    CSB-YP301809SSQ
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    聚囊藻屬rne重組蛋白
  • 貨號：
    CSB-EP301809SSQ
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    聚囊藻屬rne重組蛋白
  • 貨號：
    CSB-EP301809SSQ-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    聚囊藻屬rne重組蛋白
  • 貨號：
    CSB-BP301809SSQ
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    聚囊藻屬rne重組蛋白
  • 貨號：
    CSB-MP301809SSQ
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rne
  • Uniprot No.：
  • 別名：
    rne; slr1129; Ribonuclease E/G-like protein; RNase E/G-like protein; EC 3.1.26.-
  • 種屬：
    Synechocystis sp. (strain PCC 6803 / Kazusa)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Endoribonuclease that plays a central role in rRNA processing and mRNA decay, and probably tRNA processing. Acts on 9S rRNA (the precursor of 5S rRNA) and RNAI, a molecule that controls the replication of ColE1 plasmid. Upon expression in E.coli does not purify with endogenous degradosome proteins. Prefers 5'-monophosphorylated substrates over 5'-triphosphorylated substrates. Complements an rne temperature-sensitive mutation in E.coli, despite being considerably shorter and not able to interact with the E.coli degradosome. Cleaves AU-rich sequences in vitro, tested with psbA2 mRNA. Complements both an rne temperature-sensitive mutation and an rng deletion in E.coli. Acts in the degradation of psaL mRNA in the presence but not the absence of the sRNA PsrR1. Cleaves the rimO-crhR transcript, contributing to the very short half-life of rimO mRNA.; mRNA for psbA2, one of the core proteins in photosystem II, is degraded in the dark under control of a cis-acting AU-rich box in its 5'-UTR. RNase E cuts in this box, suggesting it is involved in this dark-induced mRNA instability.; CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Endogenous RNase E is required for correct processing of pre-crRNA for the CRISPR3 subtype III-B system in this genome (genes sll7080 to sll7095). This CRISPR3 system does not include a cas6 gene, which is the usual RNase involved in crRNA maturation.
  • 亞細胞定位：
    Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    RNase E/G family
  • 數據庫鏈接：


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