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  4. Recombinant Streptococcus pneumoniae Penicillin-binding protein 2B (penA), partial

Recombinant Streptococcus pneumoniae Penicillin-binding protein 2B (penA), partial

  • 中文名稱：
    肺炎鏈球菌penA重組蛋白
  • 貨號：
    CSB-YP363419SMP
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    肺炎鏈球菌penA重組蛋白
  • 貨號：
    CSB-EP363419SMP
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    肺炎鏈球菌penA重組蛋白
  • 貨號：
    CSB-EP363419SMP-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    肺炎鏈球菌penA重組蛋白
  • 貨號：
    CSB-BP363419SMP
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    肺炎鏈球菌penA重組蛋白
  • 貨號：
    CSB-MP363419SMP
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    penA
  • Uniprot No.：
  • 別名：
    penA; pbp2b; spr1517; Penicillin-binding protein 2B
  • 種屬：
    Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 基因功能參考文獻：
    1. The amino acid substitutions in PBP1a, PBP2b, and PBP2x were the crucial factors for beta-lactam resistance. PMID: 27042760
    2. the presence of low-affinity pbp2x enables certain pneumococcal colonies to survive in the presence of beta-lactams PMID: 24777105
    3. Depletion of PBP2b expression gave rise to long chains of cells in which individual cells were compressed in the direction of the long axis. This change is consistent with a role for pneumococcal PBP2b in the synthesis of the lateral cell wall. PMID: 23873916
    4. Mutations in pbp2b is associated with beta-lactam resistance in Streptococcus pneumoniae. PMID: 21379570
    5. Penicillin or cefotaxime resistance in S. pneumoniae in Thailand was due to affinity reduction of PBP2b, similar to changes found in other Asian isolates. PMID: 21294378
    6. Finds 7 distinct pbp2b RFLP patterns. RFLP pattern 1 was the most predominant resistant pattern. The RFLP pattern 2 was found only in the penicillin-susceptible group. PMID: 16124434
    7. Expression and limited proteolysis of a soluble form of S. pneumoniae R6 penicillin-binding protein 2B that produces the transpeptidase domain. PMID: 18391428
    8. Analysis of pbp2b genes revealed highly similar patterns of nucleotide and amino acid sequence variation among most resistant isolates, while penicillin intermediate Streptococcus pneumonia might be associated with novel gene sequence variants. PMID: 19040068
    9. Pbp2b gene is mostly present in the intermediate resistant strains PMID: 19736537

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  • 亞細胞定位：
    Cell membrane; Single-pass membrane protein.
  • 蛋白家族：
    Transpeptidase family
  • 數據庫鏈接：

    KEGG: spr:spr1517

    STRING: 171101.spr1517



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