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  4. Recombinant Staphylococcus aureus Chromosomal replication initiator protein DnaA (dnaA)

Recombinant Staphylococcus aureus Chromosomal replication initiator protein DnaA (dnaA)

  • 中文名稱：
    金黃色釀膿葡萄球菌dnaA重組蛋白
  • 貨號：
    CSB-YP646119FLF
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    金黃色釀膿葡萄球菌dnaA重組蛋白
  • 貨號：
    CSB-EP646119FLF
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    金黃色釀膿葡萄球菌dnaA重組蛋白
  • 貨號：
    CSB-EP646119FLF-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    金黃色釀膿葡萄球菌dnaA重組蛋白
  • 貨號：
    CSB-BP646119FLF
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    金黃色釀膿葡萄球菌dnaA重組蛋白
  • 貨號：
    CSB-MP646119FLF
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    dnaA
  • Uniprot No.：
  • 別名：
    dnaA; SAOUHSC_00001Chromosomal replication initiator protein DnaA
  • 種屬：
    Staphylococcus aureus (strain NCTC 8325)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-453
  • 氨基酸序列
    MSEKEIWEKV LEIAQEKLSA VSYSTFLKDT ELYTIKDGEA IVLSSIPFNA NWLNQQYAEI IQAILFDVVG YEVKPHFITT EELANYSNNE TATPKETTKP STETTEDNHV LGREQFNAHN TFDTFVIGPG NRFPHAASLA VAEAPAKAYN PLFIYGGVGL GKTHLMHAIG HHVLDNNPDA KVIYTSSEKF TNEFIKSIRD NEGEAFRERY RNIDVLLIDD IQFIQNKVQT QEEFFYTFNE LHQNNKQIVI SSDRPPKEIA QLEDRLRSRF EWGLIVDITP PDYETRMAIL QKKIEEEKLD IPPEALNYIA NQIQSNIREL EGALTRLLAY SQLLGKPITT ELTAEALKDI IQAPKSKKIT IQDIQKIVGQ YYNVRIEDFS AKKRTKSIAY PRQIAMYLSR ELTDFSLPKI GEEFGGRDHT TVIHAHEKIS KDLKEDPIFK QEVENLEKEI RNV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.
  • 基因功能參考文獻：
    1. We show that cells lacking Noc over-initiate and mutations in the initiator gene dnaA suppress this defect. Importantly, these dnaA mutations also partially suppress the division problems associated with Deltanoc. Reciprocally, we show that over-expression of DnaA enhances the over-initiation and cell division phenotypes of the Deltanoc mutant. PMID: 28723932
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    DnaA family
  • 數據庫鏈接：


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