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Recombinant Staphylococcus aureus ATP-dependent Clp protease proteolytic subunit (clpP)

  • 中文名稱:
    Recombinant Staphylococcus aureus ATP-dependent Clp protease proteolytic subunit(clpP)
  • 貨號:
    CSB-YP645172FLF
  • 說明書:
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Staphylococcus aureus ATP-dependent Clp protease proteolytic subunit(clpP)
  • 貨號:
    CSB-EP645172FLF
  • 說明書:
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Staphylococcus aureus ATP-dependent Clp protease proteolytic subunit(clpP)
  • 貨號:
    CSB-EP645172FLF-B
  • 說明書:
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Staphylococcus aureus ATP-dependent Clp protease proteolytic subunit(clpP)
  • 貨號:
    CSB-BP645172FLF
  • 說明書:
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Staphylococcus aureus ATP-dependent Clp protease proteolytic subunit(clpP)
  • 貨號:
    CSB-MP645172FLF
  • 說明書:
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    clpP
  • Uniprot No.:
  • 別名:
    clpP; SAOUHSC_00790ATP-dependent Clp protease proteolytic subunit; EC 3.4.21.92; Endopeptidase Clp
  • 種屬:
    Staphylococcus aureus (strain NCTC 8325)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-195
  • 氨基酸序列
    MNLIPTVIET TNRGERAYDI YSRLLKDRII MLGSQIDDNV ANSIVSQLLF LQAQDSEKDI YLYINSPGGS VTAGFAIYDT IQHIKPDVQT ICIGMAASMG SFLLAAGAKG KRFALPNAEV MIHQPLGGAQ GQATEIEIAA NHILKTREKL NRILSERTGQ SIEKIQKDTD RDNFLTAEEA KEYGLIDEVM VPETK
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.
  • 基因功能參考文獻:
    1. Wild-type SaClpP was found exclusively in the active extended form, with the N-terminal domains of its component protomers in predominantly beta-hairpin conformations that are less well-defined than other regions of the protein. PMID: 29941580
    2. Another important biofilm regulatory system is the ATP-dependent protease ClpP, the proteolytic subunit of Clp proteases. The clpP mutant showed significantly reduced virulence in a murine model of subcutaneous foreign body infection, indicating that the increased biofilm formation capability cannot compensate for the intrinsic functions of ClpP during infection. PMID: 28555174
    3. the hydrophobic binding pocket is a major conformational regulatory site with implications for both ClpXP proteolysis and ADEP-based anti-bacterial activity PMID: 25695750
    4. Finally, the scope of TrfA-mediated proteolysis was expanded by identifying TrfA as the adaptor that works with ClpCP to degrade antitoxins in Staphylococcus aureus. PMID: 25225270
    5. ClpP protease plays a central role in the proteolysis-related pathways of Staphylococcus aureus. PMID: 23253041
    6. The tetradecameric oligomerization state is necessary for proper formation of the active site of ClpP. PMID: 22291011
    7. results indicate a strong impact of ClpP proteolytic activity on virulence, stress response, and physiology in S. aureus PMID: 16885446

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  • 亞細胞定位:
    Cytoplasm.
  • 蛋白家族:
    Peptidase S14 family
  • 數據庫鏈接:


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