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  4. Recombinant Staphylocoagulase, partial

Recombinant Staphylocoagulase, partial

  • 中文名稱：
    金黃色釀膿葡萄球菌N/A重組蛋白
  • 貨號：
    CSB-YP325536FKZ
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    金黃色釀膿葡萄球菌N/A重組蛋白
  • 貨號：
    CSB-EP325536FKZ
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    金黃色釀膿葡萄球菌N/A重組蛋白
  • 貨號：
    CSB-EP325536FKZ-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    金黃色釀膿葡萄球菌N/A重組蛋白
  • 貨號：
    CSB-BP325536FKZ
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    金黃色釀膿葡萄球菌N/A重組蛋白
  • 貨號：
    CSB-MP325536FKZ
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 別名：
    Staphylocoagulase
  • 種屬：
    Staphylococcus aureus
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答
Q:

1. Could you please provide sequence, availability and tag information for all available sizes of all available expression hosts.
Have you received any customer feedback about the activity of the protein?
2. We also have additional questions:
- Which strain of S.aureus is the gene cloned from?
- Do you have a recombinant coagulase protein with a His-tag?
- Is the protein tested for clotting ability with rabbit for each batch you sell?

A:
Thanks for your inquiry.Recombinant Staphylococcus aureus Staphylocoagulase ,partial
CSB-YP325536FKZ >> Yeast
CSB-EP325536FKZ >> E.coli
CSB-BP325536FKZ >> Baculovirus
CSB-MP325536FKZ >> Mammalian cell
Expression Region: 27-308aa; Fragment at the N-terminal.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

IVTKDYSKESRVNENSKYGTLISDWYLKGRLTSLESQFINALDILETYHYGEKEYKDAKDKLMTRILGEDQYLLERKKVQYEEYKKLYQKYKEENPTSKGLKLKTFDQYTIEDLTMREYNELTESLKSAVKDFEKDVEKIENQHHDLKPFTDEMEEKATSRVDDLANKAYSVYFAFVRDTQHKTEALELKAKVDLVLGDEDKPHRISNERIEKEMIKDLESIIEDFFIETGLNKPGNITSYDSSKHHYKNHSEGFEALVKETREAVANADESWKTKTVKKYG


Pls feel free to contact us if you're interesed in some other expression regions, we will check if we can provide.
Sorry, we didn't receive any feedback about the activity from other customers, and also haven't tested the activity, we can't 100% guarantee its activity.
However, as we adpot affinity chromatography purification, it's purified under very mild condition, it should be active, we recommend you to purchase a small size to have a try. We can provide one literature for your reference if you need.
① Link: https://www.uniprot.org/uniprot/P17855
The derived strain is Staphylococcus aureus, but it doesn't show the specific strain.
Do you have any special requirement for the strain ? If yes, pls let us know.
② We can provide the recombinant coagulase protein with His-tag, pls check the details listed as below:
Recombinant Yersinia pestis Coagulase/fibrinolysin(pla)
CSB-YP325941YAS >>
CSB-EP325941YAS >> E.coli
CSB-BP325941YAS >> Baculovirus
CSB-MP325941YAS >> Mammalian cell
Expression Region: 21-312aa; Full length of the mature protein.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

ASSQLIPNISPDSFTVAASTGMLSGKSHEMLYDAETGRKISQLDWKIKNVAILKGDISWDPYSFLTLNARGWTSLASGSGNMDDYDWMNENQSEWTDHSSHPATNVNHANEYDLNVKGWLLQDENYKAGITAGYQETRFSWTATGGSYSYNNGAYTGNFPKGVRVIGYNQRFSMPYIGLAGQYRINDFELNALFKFSDWVRAHDNDEHYMRDLTFREKTSGSRYYGTVINAGYYVTPNAKVFAEFTYSKYDEGKGGTQTIDKNSGDSVSIGGDAAGISNKNYTVTAGLQYRF


Regarding the requirement for the His-Tag, do you mean only the His-Tag, no other tag at the other end ?
Which terminus ? His-Tag at the N-terminal or C-terminal ? Is it 6xHis or 10xHis ?
The expected tag for each expression system is listed as above, we can try to express your specified tag preferentially, if it fails, we will communicate with you in advance, and provide other tag.
③Sorry, we don't test this protein for clotting ability with rabbit, and we also haven't tested the activity.

靶點詳情

  • 功能：
    Staphylocoagulase is an extracellular protein which specifically forms a complex with human prothrombin. This complex named staphylothrombin can clot fibrinogen without any proteolytic cleavage of prothrombin.
  • 蛋白家族：
    Staphylocoagulase family


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