Recombinant Shigella flexneri Outer membrane protein IcsA autotransporter (icsA), partial

中文名稱(chēng)：

弗累克斯訥氏桿菌icsA重組蛋白
貨號(hào)：

CSB-YP745216SZB
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱(chēng)：

弗累克斯訥氏桿菌icsA重組蛋白
貨號(hào)：

CSB-EP745216SZB
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱(chēng)：

弗累克斯訥氏桿菌icsA重組蛋白
貨號(hào)：

CSB-EP745216SZB-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱(chēng)：

弗累克斯訥氏桿菌icsA重組蛋白
貨號(hào)：

CSB-BP745216SZB
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱(chēng)：

弗累克斯訥氏桿菌icsA重組蛋白
貨號(hào)：

CSB-MP745216SZB
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

icsA
Uniprot No.：

Q7BCK4
別名：

icsA; virG; CP0182; Outer membrane protein IcsA autotransporter [Cleaved into: Outer membrane protein IcsA; Outer membrane protein IcsA translocator]
種屬：

Shigella flexneri
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Essential for bacterial spreading by eliciting polar deposition of filamentous actin (actin-based motility). Inside the host cell mediates nucleation and polymerization of actin molecules on the bacterial surface, which provides the propulsive force for intracellular movement and intercellular dissemination of the bacterium. During invasion of mammalian cells, triggers autophagy by binding to APG5L. Interaction with IcsB leads to escape from the autophagic host defense system. Also binds ATP and displays weak ATPase activity.
基因功能參考文獻(xiàn)：
1. Identification of the H-NS and VirF-binding sites on the icsA promoter regions. PMID: 21724612
2. Data identified a putative autochaperone region required for IcsA biogenesis, and our data suggest an additional region, not previously identified, is required for N-WASP recruitment. PMID: 18456802
亞細(xì)胞定位：

[Outer membrane protein IcsA autotransporter]: Periplasm.; [Outer membrane protein IcsA]: Secreted. Cell surface. Note=The alpha domain is localized as a cap over the old pole of the bacterial surface.; [Outer membrane protein IcsA translocator]: Cell outer membrane; Multi-pass membrane protein. Note=The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane.
數(shù)據(jù)庫(kù)鏈接：

KEGG: sfl:CP0182