Recombinant Shigella flexneri Glutaredoxin-4 (grxD)

^{In Stock}

中文名稱：

弗累克斯訥氏桿菌grxD重組蛋白
貨號：

CSB-EP364758SZB
規格：

￥2328
圖片：
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP364758SZB could indicate that this peptide derived from E.coli-expressed Shigella flexneri grxD.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP364758SZB could indicate that this peptide derived from E.coli-expressed Shigella flexneri grxD.
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產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

grxD
Uniprot No.：

P0AC72
別名：

grxD; ydhD; SF1682; S1814Glutaredoxin 4; Grx4; Monothiol glutaredoxin
種屬：

Shigella flexneri
蛋白長度：

Full Length
來源：

E.coli
分子量：

28.9 kDa
表達區域：

1-115aa
氨基酸序列

MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACGERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKETAAKYKSEEPDAE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer，50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

This Recombinant Shigella flexneri grxD protein was made through genetic engineering. By putting the grxD gene into the genetic material of E.coli cell, the E.coli could be used as factories or producers to make the desired grxD protein for research uses. The expression region of this protein is at 1-115aa. N-terminal 6xHis-SUMO tag was used in the expression process. The purity is 85%+ determined by SDS-PAGE.
The cytosolic monothiol glutaredoxin GrxD plays a role in adaptation to iron starvation during infection. GrxD is repressed by the transcription factor SreA in iron replete conditions. During iron starvation, GrxD displays predominant nuclear localization. Downregulation of GrxD expression results in de-repression of genes involved in iron-dependent pathways and repression of genes involved in iron acquisition during iron starvation, but did not significantly affect these genes during iron sufficiency. GrxD displays protein-protein interaction with components of the cytosolic iron-sulfur cluster biosynthetic machinery, indicating a role in this process, and with the transcription factors SreA and HapX, which mediate iron regulation of iron acquisition and iron-dependent pathways. There is a GrxD-independent mechanism for sensing iron sufficiency by HapX. Inactivation of SreA suppresses the lethal effect caused by GrxD inactivation. Taken together, this study demonstrates that GrxD is crucial for iron homeostasis in A. fumigatus.
Datasheet & COA：

Please contact us to get it.