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  4. Recombinant Sheep Steroid 17-alpha-hydroxylase/17,20 lyase (CYP17A1), partial

Recombinant Sheep Steroid 17-alpha-hydroxylase/17,20 lyase (CYP17A1), partial

  • 中文名稱：
    Recombinant Sheep Steroid 17-alpha-hydroxylase/17,20 lyase(CYP17A1) ,partial
  • 貨號：
    CSB-YP643683SH
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Sheep Steroid 17-alpha-hydroxylase/17,20 lyase(CYP17A1) ,partial
  • 貨號：
    CSB-EP643683SH
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Sheep Steroid 17-alpha-hydroxylase/17,20 lyase(CYP17A1) ,partial
  • 貨號：
    CSB-EP643683SH-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Sheep Steroid 17-alpha-hydroxylase/17,20 lyase(CYP17A1) ,partial
  • 貨號：
    CSB-BP643683SH
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Sheep Steroid 17-alpha-hydroxylase/17,20 lyase(CYP17A1) ,partial
  • 貨號：
    CSB-MP643683SH
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    CYP17A1; CYP17Steroid 17-alpha-hydroxylase/17,20 lyase; EC 1.14.14.19; 17-alpha-hydroxyprogesterone aldolase; EC 1.14.14.32; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; Steroid 17-alpha-monooxygenase
  • 種屬：
    Ovis aries (Sheep)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol. Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione. Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
  • 基因功能參考文獻：
    1. Two isoforms for sheep cytochrome P450 17alpha-hydroxylase/17,20-lyase (CYP17) differ by 2 nucleotides. This study investigates the effect of these differences on cortisol production as a function of hypothalamic-pituitary-adrenal axis activity. PMID: 23243163
    2. Data show that androgenization had effect on a reduction in the expression of StAR, CYP11A, CYP17, and LHR at d 90 of gestation. PMID: 21325046
  • 亞細胞定位：
    Endoplasmic reticulum membrane. Microsome membrane.
  • 蛋白家族：
    Cytochrome P450 family
  • 數據庫鏈接：

    KEGG: oas:493968

    UniGene: Oar.399



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