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  4. Recombinant Sheep FMRFamide-related peptides (NPVF)

Recombinant Sheep FMRFamide-related peptides (NPVF)

  • 中文名稱：
    綿羊NPVF重組蛋白
  • 貨號：
    CSB-YP016032SH
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    綿羊NPVF重組蛋白
  • 貨號：
    CSB-EP016032SH
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    綿羊NPVF重組蛋白
  • 貨號：
    CSB-EP016032SH-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    綿羊NPVF重組蛋白
  • 貨號：
    CSB-BP016032SH
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    綿羊NPVF重組蛋白
  • 貨號：
    CSB-MP016032SH
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NPVF
  • Uniprot No.：
  • 別名：
    NPVF; RFRP; Pro-FMRFamide-related neuropeptide VF; FMRFamide-related peptides) [Cleaved into: Neuropeptide NPSF; Neuropeptide RFRP-1; Neuropeptide RFRP-2; Neuropeptide NPVF; Neuropeptide RFRP-3)]
  • 種屬：
    Ovis aries (Sheep)
  • 蛋白長度：
    Cytoplasmic domain
  • 表達區域：
    58-92
  • 氨基酸序列
    SLT FEEVKDWGPK IKMNTPAVNK MPPSAANLPL RF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Neuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP, but RFRP-2 shows no inhibitory activity. Neuropeptide NPVF blocks morphine-induced analgesia. May act in concert with kisspeptin, through opposing affects, to regulate the activity of gonadotropin-releasing hormone (GnRH) neurons across the seasons, leading to an annual change in fertility and the cyclical seasonal transition from non-breeding to breeding season.
  • 基因功能參考文獻：
    1. These results suggested that the changes of RFRP-3 in response to food restriction might influence the hypothalamic-pituitary-ovarian axis and inhibit ovarian development. PMID: 25039406
    2. GnIH/RFRP system is not activated by psychosocial stress in ewes, suggesting that it is an unlikely mediator of the effects of stress on LH secretion. PMID: 21073555
    3. Kisspeptin and RFRP, act in concert, with opposing effects, to regulate the activity of GnRH neurons across the seasons, leading to the annual change in fertility and the cyclical seasonal transition from nonbreeding to breeding season. PMID: 18617612
    4. RFRP-3 is a specific and potent mammalian gonadotropin-inhibiting hormone, that acts upon pituitary gonadotropes to reduce GnRH-stimulated gonadotropin secretion. PMID: 18617613
    5. Results describe the cloning of ovine hypothalamic RFamide-related peptide cDNA and its cognate receptor, NPFF1, and their photoperiodic expression pattern in sheep hypothalamus. PMID: 18752651
    6. Data suggest that RFRP-3 neurones project to hypothalamic regions and cells involved in regulation of energy balance and reproduction in the ovine brain. PMID: 19500220

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    FARP (FMRFamide related peptide) family
  • 組織特異性：
    Expressed in hypothalamus, where it is localized to the dorsomedial hypothalamic nucleus (DMH), paraventricular nucleus (PVN), and to neuronal projections from the PVN to the neurosecretory zone of the median eminence.
  • 數據庫鏈接：

    KEGG: oas:100127217

    UniGene: Oar.14526



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