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  4. Recombinant Serine/threonine-protein kinase plk-1 (plk-1), partial

Recombinant Serine/threonine-protein kinase plk-1 (plk-1), partial

  • 中文名稱：
    秀麗隱桿線蟲plk-1重組蛋白
  • 貨號：
    CSB-YP339442CXY
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲plk-1重組蛋白
  • 貨號：
    CSB-EP339442CXY
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲plk-1重組蛋白
  • 貨號：
    CSB-EP339442CXY-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲plk-1重組蛋白
  • 貨號：
    CSB-BP339442CXY
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲plk-1重組蛋白
  • 貨號：
    CSB-MP339442CXY
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    plk-1
  • Uniprot No.：
  • 別名：
    plk-1; plc1; C14B9.4; Serine/threonine-protein kinase plk-1; EC 2.7.11.21; Polo-like kinase 1
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for oocyte nuclear envelope breakdown before entry of oocyte into spermatheca. In meiotic cells, required for spindle dynamics and probably for spindle attachment to the chromosomes. Zygotic role in the development of the germline and nerve cord. In mitotic cells, plays a role in spindle organization and centrosome maturation. Involved in asymmetric nuclear localization of cdc-25.1 during embryogenesis which affects cell division timing. Together with plk-2, regulates cytoplasm polarity in early embryos. May play a minor role in chromosome pairing and synapsis during oocyte meiosis I.
  • 基因功能參考文獻：
    1. PLK1 inhibition mitigates autophagy in cancer cells both under nutrient starvation and sufficiency, and a role of PLK1 in autophagy is also observed in the invertebrate model organism Caenorhabditis elegans. PMID: 28102733
    2. These results reveal a crucial and conserved role of phosphorylation of the N terminus of Bora for Plk1 activation and mitotic entry. PMID: 27068477
    3. Our data further show that PLK-1 is needed for nuclear envelope breakdown during early embryogenesis PMID: 26490119
    4. PLK-1 substitutes for Mps1 in controlling spindle checkpoint initiation in C. elegans. PMID: 26119738
    5. NCAPG2 plays an important role in regulating proper chromosome segregation through a functional interaction with PLK1 during mitosis PMID: 25109385
    6. The result provide key insights into the regulation of homolog pairing and reveal that targeting of plk-1 to the NE by meiotic chromosomes establishes the conserved linkages to cytoskeletal forces needed for homology assessment. PMID: 22018921
    7. plk-1 asymmetry contributes to asynchronous cell division in C. elegans embryos. PMID: 18305005

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Midbody. Cytoplasm. Nucleus. Chromosome. Chromosome, centromere, kinetochore.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily
  • 組織特異性：
    Embryos.
  • 數據庫鏈接：

    KEGG: cel:CELE_C14B9.4

    STRING: 6239.C14B9.4b

    UniGene: Cel.18095



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