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  4. Recombinant Schizosaccharomyces pombe Tip elongation aberrant protein 1 (tea1), partial

Recombinant Schizosaccharomyces pombe Tip elongation aberrant protein 1 (tea1), partial

  • 中文名稱：
    龐貝裂殖酵母tea1重組蛋白
  • 貨號：
    CSB-YP308936SXV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母tea1重組蛋白
  • 貨號：
    CSB-EP308936SXV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母tea1重組蛋白
  • 貨號：
    CSB-EP308936SXV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母tea1重組蛋白
  • 貨號：
    CSB-BP308936SXV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母tea1重組蛋白
  • 貨號：
    CSB-MP308936SXV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    tea1
  • Uniprot No.：
  • 別名：
    tea1; alp8; SPCC1223.06; Tip elongation aberrant protein 1; Altered polarity protein 8; Cell polarity protein tea1
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Cell polarity protein. Acts as an end marker, directing the growth machinery to the cell poles. Involved in the regulation of microtubular organization, affecting the maintenance of a single central axis. Prevents the curling of microtubule tips around the cell ends and is required for the retention of polarity factors such as pom1, tip1 and tea2 at the cell ends, necessary for the cell to grow in a straight line. Links tip1 and tea4 in a common complex.
  • 基因功能參考文獻：
    1. Study proposes that Cki3 monitors the timing of growth polarity transition from monopolar to bipolar during G2 phase onset through Tea1 phosphorylation by regulating the interaction between Tea1 and Tea4. PMID: 26525038
    2. Study constructed fission yeast strains exhibiting depressed microtubule dynamics by mutating the beta-tubulin gene, nda3. This interfered with efficient accumulation of a polarity factor Tea1 at cell tips. PMID: 21548112
    3. Data suggest that rather than acting simply as a Tea1 receptor or as a molecular "glue" to retain Tea1, Mod5 functions catalytically to stimulate incorporation of Tea1 into a stable tip-associated cluster network. PMID: 20850323
    4. End4/Sla2 acts downstream of the polarity marker Tea1 and is implicated in the recruitment of the actin cytoskeleton to bring about polarised cell growth PMID: 15827087
    5. results suggest that independent protein-protein interactions among tea1p, tea3p and mod5p collectively contribute to tea1p anchoring at cell tips via a multistep and multimode mechanism PMID: 16222337
    6. nuclear centering becomes essential when Pom1/Tea1/Tea4 function is compromised and that Kin1 expression level is a key regulatory element in this situation PMID: 19597328

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton. Note=Present at both poles of the cell throughout the cell cycle whether they are growing or not. Located at the ends of microtubules growing towards the cell poles. An intact microtubular skeleton is required to maintain the location at the cell tips; on the other hand, a normally organized actin cytoskeleton is not required.
  • 數據庫鏈接：


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