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  4. Recombinant Schizosaccharomyces pombe Serine/threonine-protein kinase gcn2 (gcn2), partial

Recombinant Schizosaccharomyces pombe Serine/threonine-protein kinase gcn2 (gcn2), partial

  • 中文名稱：
    龐貝裂殖酵母gcn2重組蛋白
  • 貨號(hào)：
    CSB-YP864062SXV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母gcn2重組蛋白
  • 貨號(hào)：
    CSB-EP864062SXV
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母gcn2重組蛋白
  • 貨號(hào)：
    CSB-EP864062SXV-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母gcn2重組蛋白
  • 貨號(hào)：
    CSB-BP864062SXV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母gcn2重組蛋白
  • 貨號(hào)：
    CSB-MP864062SXV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    gcn2
  • Uniprot No.：
  • 別名：
    gcn2; ppk28; SPBC36B7.09; SPBP18G5.01; eIF-2-alpha kinase GCN2; Serine/threonine-protein kinase gcn2; EC 2.7.11.1; Serine/threonine-protein kinase ppk28
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/tif211) on 'Ser-52' in response to low amino acid availability. Plays a role as an activator of the general amino acid control (GAAC) pathway required for adapatation to amino acid starvation. Converts phosphorylated eIF-2-alpha/tif211 either to a competitive inhibitor of translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator fil1, and hence allowing fil1-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs.
  • 基因功能參考文獻(xiàn)：
    1. Gcn2 and eIF2a phosphorylation are involved in the modulation of G1 phase cell cycle arrest, cell survival and mating under nutritional stress in the fission yeast Schizosaccharomyces pombe. PMID: 23687372
    2. S. pombe Cpc2 facilitates the GAAC response through the regulation of Gcn2 activation PMID: 23671279
    3. Gcn2 is the primary eIF2 kinase induced during a nutrient downshift, treatment with the amino acid biosynthetic inhibitor 3-aminotriazole or upon exposure to high NaCl concentrations. It is tandemly activated with Hri2 in response to H2O2 exposure. [Gcn2] PMID: 15611163
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily
  • 數(shù)據(jù)庫(kù)鏈接：


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