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  4. Recombinant Schizosaccharomyces pombe Protein kinase rad3 (rad3), partial

Recombinant Schizosaccharomyces pombe Protein kinase rad3 (rad3), partial

  • 中文名稱：
    龐貝裂殖酵母rad3重組蛋白
  • 貨號：
    CSB-YP311582SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rad3重組蛋白
  • 貨號：
    CSB-EP311582SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rad3重組蛋白
  • 貨號：
    CSB-EP311582SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rad3重組蛋白
  • 貨號：
    CSB-BP311582SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rad3重組蛋白
  • 貨號：
    CSB-MP311582SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rad3
  • Uniprot No.：
  • 別名：
    rad3; SPBC216.05; Protein kinase rad3; EC 2.7.11.1; DNA repair protein rad3
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Serine/threonine kinase which activates checkpoint signaling upon genotoxic stresses. Involved in G2 arrest following DNA damage where it phosphorylates chk1. Phosphorylation of 'Thr-73' and 'Ser-80' of checkpoint mediator crb2 promotes its interaction with chk1. It is also involved in the dependence of mitosis on the completion of DNA replication.
  • 基因功能參考文獻：
    1. fission yeast shelterin maintains telomere length homeostasis by coordinating the differential arrival of leading (Polepsilon) and lagging (Polalpha) strand DNA polymerases at telomeres to modulate Rad3(ATR) association PMID: 24244195
    2. Rad3 phosphorylates histone H2A to mobilize Brc1 to critical genomic domains during S-phase, and this pathway functions in parallel with Rqh1 DNA helicase in maintaining genome integrity. PMID: 20661445
    3. Rad3 is known to play an essential role in coordination of DNA replication and cell division during both mitotic growth and meiosis. PMID: 16286472
    4. DNA replication is stalled leads to release of Rad3 and Rad26 from chromatin and entry into an aberrant mitosis. PMID: 17531813
    5. An elevated level of Cdc18 activates the Rad3-dependent checkpoint either directly or indirectly. PMID: 17690116

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    PI3/PI4-kinase family, ATM subfamily
  • 數據庫鏈接：


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