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  4. Recombinant Schizosaccharomyces pombe Protein Dicer (dcr1), partial

Recombinant Schizosaccharomyces pombe Protein Dicer (dcr1), partial

  • 中文名稱(chēng)：
    Recombinant Schizosaccharomyces pombe Protein Dicer(dcr1) ,partial
  • 貨號(hào)：
    CSB-YP600673SXV
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Schizosaccharomyces pombe Protein Dicer(dcr1) ,partial
  • 貨號(hào)：
    CSB-EP600673SXV
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Schizosaccharomyces pombe Protein Dicer(dcr1) ,partial
  • 貨號(hào)：
    CSB-EP600673SXV-B
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    Recombinant Schizosaccharomyces pombe Protein Dicer(dcr1) ,partial
  • 貨號(hào)：
    CSB-BP600673SXV
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Schizosaccharomyces pombe Protein Dicer(dcr1) ,partial
  • 貨號(hào)：
    CSB-MP600673SXV
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    dcr1
  • Uniprot No.：
  • 別名：
    dcr1; SPCC188.13c; SPCC584.10cProtein Dicer; Cell cycle control protein dcr1; RNA interference pathway protein dcr1) [Includes: Endoribonuclease dcr1; EC 3.1.26.-); ATP-dependent helicase dcr1; EC 3.6.4.-)]
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Required for G1 arrest and mating in response to nitrogen starvation. Ago1 regulation of cytokinesis and cell cycle checkpoints occurs downstream of dcr1. Required, indirectly, for regulated hyperphosphorylation of cdc2.; Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation, accurate chromosome segregation, centromere cohesion and telomere function during mitosis and meiosis. Digests double-stranded RNA (dsRNA) producing 21 to 23 bp dsRNAs, so-called interfering RNAs (siRNA). Required for both post-transcriptional and transcriptional gene silencing. Required for silencing at the centromeres and for initiation of transcriptionally silent heterochromatin at the mating type locus. Promotes histone H3 'Lys-10' methylation necessary for centromere function. Required for recruitment of swi6 and cohesin to an ectopic dg repeat.
  • 基因功能參考文獻(xiàn)：
    1. Findings suggest that in S. pombe Dcr1 has a genome-wide role in terminating transcription by releasing Pol II at sites of collision between transcription and replication and thus maintains genome stability. PMID: 25417108
    2. Studies indicate that S. pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1). PMID: 24462781
    3. Data indicate that Argonaute, loaded with longer RNA precursors, recruits Triman (SPBC29A10.09c, 3'-5' Exonuclease) to generate dicer-independent priRNAs mature priRNAs and siRNAs. PMID: 24095277
    4. these results reveal novel insights into the mechanism of nuclear retention of Dcr1 and raise the possibility that this new class of dsRBDs might generally function in nucleocytoplasmic trafficking and not substrate binding PMID: 21847092
    5. Dcr1 has a role in post-transcriptional regulation of gene expression in Schizosaccharomyces pombe [review] PMID: 17981703

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Helicase family, Dicer subfamily
  • 數(shù)據(jù)庫(kù)鏈接：


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