Recombinant Schizosaccharomyces pombe Protection of telomeres protein 1 (pot1), partial

中文名稱：

龐貝裂殖酵母pot1重組蛋白
貨號：

CSB-YP018382SXV
規(guī)格：
來源：

Yeast
其他：

中文名稱：

龐貝裂殖酵母pot1重組蛋白
貨號：

CSB-EP018382SXV
規(guī)格：
來源：

E.coli
其他：

中文名稱：

龐貝裂殖酵母pot1重組蛋白
貨號：

CSB-EP018382SXV-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

龐貝裂殖酵母pot1重組蛋白
貨號：

CSB-BP018382SXV
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

龐貝裂殖酵母pot1重組蛋白
貨號：

CSB-MP018382SXV
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

pot1
Uniprot No.：

O13988
別名：

pot1; SPAC26H5.06; Protection of telomeres protein 1
種屬：

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length.
基因功能參考文獻：
1. Thus, our results suggest that pik1(+) is required for the maintenance of circular chromosomes. PMID: 29410177
2. The Pot1 DNA-binding domain is capable of binding DNA in at least two structurally distinct binding modes. PMID: 25074378
3. Pot1 promiscuity is implemented through binding modes that thermodynamically compensate for base-substitutions through alternate stacking interactions and hydrogen-bonding networks. PMID: 23201273
4. Results indicate an unanticipated ssDNA-binding mode in which two SpPot1 molecules bind an oligonucleotide containing two telomeric repeats. PMID: 21911358
5. The high affinity conserved DNA-binding activity of Pot1 protein is conferred by a DNA-binding domain consisting of two subdomains. PMID: 21815629
6. The Pot1 (protection of telomeres) protein prevents access of telomerase to the 3'-ends of telomeres and protects telomeric DNA against exonucleolytic degradation. [pot1] PMID: 15637058
7. Pot1(1-389) presents a new model for studying and understanding the ssDNA-binding activity of S. pombe Pot1 and illustrates the malleability of both the ssDNA ligand and the oligosaccharidce-binding fold in the recognition and binding of telomeric ssDNA. PMID: 16842820
8. Considering that the antiparallel tetraplex inhibits telomerase-mediated telomere elongation, we conclude that the ability of Pot1 to unfold the antiparallel tetraplex is required for telomerase-mediated telomere regulation. PMID: 17158862
9. Characterization of the thermodynamics and accommodation of both cognate and noncognate telomeric single-stranded DNA (ssDNA) sequences by Pot1pN, an autonomous ssDNA-binding domain (residues 1-187) found in full-length S. pombe Pot1. PMID: 18355038
10. study shows Tpz1 forms a complex with Pot1; Tpz1 binds to Ccq1 & Poz1 which protect telomeres redundantly & regulate telomerase in positive & negative manners; thus, Pot1-Tpz1 complex accomplishes its functions by recruiting effector molecules Ccq1 & Poz1 PMID: 18535244
11. model in which conformational selection and induced fit play important roles in the recognition of ssDNA by Pot1pN (residues 1-187 of full-length Pot1) PMID: 19232358
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亞細胞定位：

Nucleus. Chromosome, telomere.
蛋白家族：

Telombin family
數(shù)據(jù)庫鏈接：

KEGG: spo:SPAC26H5.06

STRING: 4896.SPAC26H5.06.1