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  4. Recombinant Schizosaccharomyces pombe Peptide-N (4)- (N-acetyl-beta-glucosaminyl)asparagine amidase

Recombinant Schizosaccharomyces pombe Peptide-N (4)- (N-acetyl-beta-glucosaminyl)asparagine amidase

  • 中文名稱：
    龐貝裂殖酵母png1重組蛋白
  • 貨號：
    CSB-YP525600SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母png1重組蛋白
  • 貨號：
    CSB-EP525600SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母png1重組蛋白
  • 貨號：
    CSB-EP525600SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母png1重組蛋白
  • 貨號：
    CSB-BP525600SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母png1重組蛋白
  • 貨號：
    CSB-MP525600SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    png1
  • Uniprot No.：
  • 別名：
    png1; SPBC1709.14; Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; PNGase; EC 3.5.1.52; Peptide:N-glycanase 1
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-333
  • 氨基酸序列
    MDFHAISQRF IDMMRSKNSQ NASQPPETYP FYHEVRQMSQ HPWMYEDPEL QDYALSILPL DKLFQDASEL EKEGDGSWGY QDYVIQALLK WFKREFFVWV NQPPCEKCGG ETHMTGNGPP NEEEKWNGVR NVELYQCNVC GHNQRFPRYN RIRALLDSRK GRCGEWANCF TFLCRALGSR ARWIWNAEDH VWTEVYSNKQ QRWVHVDSGE ESFDEPLIYE QGWGKKMSYC LGFGIDSVRD VSHRYIRHPE NGLPRDRCPE SVLQQALHEI NIEFRSRLTD SERKALEEED KREKDELDGY MRPVSQATPT NTDLPARQTG NVEWKEKRGE AGK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.
  • 基因功能參考文獻：
    1. N-terminal alpha-helix of SpPNGase was responsible for the protein-protein interaction. we suggest that the N-terminal alpha-helices of PNGase are derived from evolutionary motif/peptide fusion. PMID: 18279662
  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Transglutaminase-like superfamily, PNGase family
  • 數據庫鏈接：


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