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  4. Recombinant Schizosaccharomyces pombe Mitogen-activated protein kinase spm1 (spm1)

Recombinant Schizosaccharomyces pombe Mitogen-activated protein kinase spm1 (spm1)

  • 中文名稱：
    龐貝裂殖酵母spm1重組蛋白
  • 貨號：
    CSB-YP842110SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母spm1重組蛋白
  • 貨號：
    CSB-EP842110SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母spm1重組蛋白
  • 貨號：
    CSB-EP842110SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母spm1重組蛋白
  • 貨號：
    CSB-BP842110SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母spm1重組蛋白
  • 貨號：
    CSB-MP842110SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    spm1
  • Uniprot No.：
  • 別名：
    spm1; pmk1; SPBC119.08; Mitogen-activated protein kinase spm1; MAP kinase spm1; EC 2.7.11.24; MAP kinase pmk1
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-422
  • 氨基酸序列
    MDRRHRVYRV FNQEMYVEPN FKVVKELGQG AYGIVCAARN VASKDQEAVA IKKITNVFSK SILTKRALRE IKLLIHFRNH RNITCIYDLD IINPYNFNEV YIYEELMEAD LNAIIKSGQP LTDAHFQSFI YQILCGLKYI HSANVIHRDL KPGNLLVNAD CELKICDFGL ARGCSENPEE NPGFMTEYVA TRWYRAPEIM LSFSSYHKGI DVWSVGCILA ELLGGTPLFK GKDFVHQLNL ILHQLGTPDE ETLSHISSSR AQEYVRSLPK QRPIPFETNF PKANPLALDL LAKLLAFDPN RRISVDDALE HPYLAVWHDP SDEPVCDSVF DFSFEYIEDA NELRRVILDE VLNFRQKVRR RSHPTNPTVN IPQPAQTVPS NDNGSFNVSS SSSSQTSNKK RHDHSYNETA AIDHKSDDNR HN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Regulates cell integrity and functions coordinately with the protein kinase C pathway (pck1 and pck2). Involved the regulation of wall architecture, cell shape, cytokinesis in exponential and stationary phase, and metabolism of ions.
  • 基因功能參考文獻：
    1. Threonine-monophosphorylated Pmk1 was able to execute most of the biological functions of the dually phosphorylated kinase. PMID: 26432170
    2. T842 phosphorylation is not essential for Pmk1 activation, and putative phosphorylation at T846 might play an additional role in Pck2 catalytic activation and downstream signaling PMID: 25416816
    3. Consistently, the phosphorylation of Pmk1 MAPK on heat shock was decreased in the cwg2-v2 mutants, and rho4- and rho5-null cells. PMID: 25651781
    4. Data indicate that mutation of any of the two first amino acids in the IYT motif led to a failure of inactive dual-specificity MAPK phosphatases Sdp1 to trap mitogen-activated protein kinase Slt2. PMID: 24465549
    5. Results suggest that Activation of Atf1 is not fully dependent on Sty1 and Pmk1. PMID: 22661707
    6. down-regulation of nuclear Pmk1 by MAPK phosphatases induced by the stress activated protein kinase pathway is important for the fine modulation of extranuclear Pmk1 activity. PMID: 22685296
    7. Results suggest that Ecm33 gene expression is regulated by Atf1 and Mbx1, and taht Ecm33 is involved in the negative feedback regulation of Pmk1 cell integrity signaling and is linked to cellular Ca(2+) signaling. PMID: 20032302
    8. analysis of the Pmk1 cell integrity pathway in Schizosaccharomyces pombe PMID: 16291757
    9. These results demonstrate that Cpp1 is a key component of the Pck2-Pmk1 signaling through the spatial control of the small GTPase Rho2. PMID: 17005909
    10. the stress-activated protein kinase (SAPK) pathway and its main effector, Sty1p MAPK, are essential for proper deactivation of Pmk1p under hypertonic stress in a process regulated by Atf1p transcription factor PMID: 17761528
    11. Atf1 is a key component of the cell integrity signaling downstream of Pmk1 MAPK PMID: 17881729
    12. Pmk1 mitogen-activated protein kinase (MAPK) directly phosphorylates Nrd1, thereby negatively regulating the binding activity of Nrd1 to Cdc4 mRNA. PMID: 19279143

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  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 數據庫鏈接：


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