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  4. Recombinant Schizosaccharomyces pombe Microtubule protein alp7 (alp7)

Recombinant Schizosaccharomyces pombe Microtubule protein alp7 (alp7)

  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Microtubule protein alp7(alp7)
  • 貨號：
    CSB-YP892507SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Microtubule protein alp7(alp7)
  • 貨號：
    CSB-EP892507SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Microtubule protein alp7(alp7)
  • 貨號：
    CSB-EP892507SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Microtubule protein alp7(alp7)
  • 貨號：
    CSB-BP892507SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Microtubule protein alp7(alp7)
  • 貨號：
    CSB-MP892507SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    alp7
  • Uniprot No.：
  • 別名：
    alp7; mia1; SPAC890.02c; Microtubule protein alp7; Altered polarity protein 7; Transforming acidic coiled-coil protein mia1; TACC protein mia1
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-474
  • 氨基酸序列
    MSDIVSSSTD YSRRSPSSSS IGTNETDHTG FHEKRQGASS ESLIPPAQRS SEESMPAPKL FPKLTSKPNP QLNLKDTLNK RVSDRLQALE LNKSFDFSGT PRPMHPISHP LSQHKTPEFK HRKRNVESIL TPKNPSLFSS SNAASQRGSL NTAPSNFAYS HSSSLQTSAS SRPPVLSNGS FPRQTNTAPL NPPVHLKDNI RNSATPSTSQ ADIPTQYPIN STQKQQAKYE AEIEGYKAKL AGTYHEISVL QNTIVNVSGQ LIAVNDQLQQ LRSGKASTSP STKDTNMRLV EGHNEETLAL QRGKYTQEEV DKLIQERMEK VAEDLHAQYS AKHTQKINAF KANYARKYEA TIQELQNQIG TAPNAPKISN SNWEEERRAL KADNQTLQKQ LEKAIQERQD MSDFLNNFKA DMAKSDKLLM QQQSQQTGDL ETLRLQLQAL QEELRVEREE RQQLIQMSED LVIAMDQLNL EQKS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for bipolar spindle formation and proper chromosome segregation. Has an indirect role in connecting the kinetochores and the plus end of pole to chromosome microtubules by targeting alp14 to the spindle pole body. Involved in the emergence of large microtubule organizing centers (MTOC) in interphase cells. Attaches to the minus ends of microtubules and associates with the sites of microtubule attachment on the nuclear envelope. This leads to the stabilzation of the microtubule bundles.
  • 基因功能參考文獻：
    1. clustered five amino acid residues within the TACC domain required for spindle pole body localization PMID: 24937146
    2. Csi1p serves as a linking molecule tethering spindle-stabilizing factors such as alp7p to the spindle pole body for promoting bipolar spindle assembly. PMID: 25057016
    3. Mia1p/Alp7p, a transforming acidic coiled-coil (TACC) protein, functions as a length-independent MT crosslinker. PMID: 19879140
    4. When the Crm1p-dependent nuclear export signal of Mia1p is disabled, Mia1p accumulates in the nucleus while its partner protein Alp14p/TOG is restricted to the cytoplasm PMID: 19606211
    5. results indicate that the spatiotemporal regulation of microtubule formation is established by the Alp7/TACC-Alp14/TOG complex through the coordinated interplay of Ran and cyclin-dependent kinase. PMID: 19696784

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  • 亞細胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Associated with the equatorial MTOC, spindle midzones, spindle pole body and mitotic kinetochore periphery. Spindle and kinetochore localization is alp14-dependent.
  • 數據庫鏈接：


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