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  4. Recombinant Schizosaccharomyces pombe Microtubule integrity protein mal3 (mal3)

Recombinant Schizosaccharomyces pombe Microtubule integrity protein mal3 (mal3)

  • 中文名稱：
    龐貝裂殖酵母mal3重組蛋白
  • 貨號：
    CSB-YP611912SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母mal3重組蛋白
  • 貨號：
    CSB-EP611912SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母mal3重組蛋白
  • 貨號：
    CSB-EP611912SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母mal3重組蛋白
  • 貨號：
    CSB-BP611912SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母mal3重組蛋白
  • 貨號：
    CSB-MP611912SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mal3
  • Uniprot No.：
  • 別名：
    mal3; SPAC18G6.15Microtubule integrity protein mal3
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-308
  • 氨基酸序列
    MSESRQELLA WINQVTSLGL TRIEDCGKGY AMIQIFDSIY QDIPLKKVNF ECNNEYQYIN NWKVLQQVFL KKGIDKVVDP ERLSRCKMQD NLEFVQWAKR FWDQYYPGGD YDALARRGNR GPANTRVMNS SAGATGPSRR RQVSSGSSTP SMTKSSANNN NVSSTANTAA VLRAKQAQQQ ITSLETQLYE VNETMFGLER ERDFYFNKLR EIEILVQTHL TTSPMSMENM LERIQAILYS TEDGFELPPD QPADLTTALT DHDTNNVAEE AQMTDLKDSE TQRVPSAPDF VHARLQSLEV DDDENITF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    May play a role in regulating the integrity of microtubules possibly by influencing their stability. Involved in an anchoring mechanism to maintain tea2 and tip1 at growing microtubule ends. Strongly stimulates the ATPase activity of tea2.
  • 基因功能參考文獻：
    1. Microtubule-bound microtubule-associated proteins Mal3 (Mal3) shows that Mal3 makes a distinctive footprint on the microtubule lattice. PMID: 29235477
    2. we show that Mal3, the fission yeast member of the EB1 family of conserved microtubule plus-end tracking proteins, is required for karyogamy, oscillatory nuclear movement, and proper segregation of chromosomes during meiosis PMID: 24196444
    3. Mal3 deletion mutant cells have a disordered cytoplasmic microtubule structure during karyogamy and initiate meiosis before completion of karyogamy. PMID: 23755176
    4. Using cryo-electron microscopy, subnanometer single-particle reconstruction, and fluorescence imaging, study presents a pseudoatomic model of how the calponin homology (CH) domain of the fission yeast EB Mal3 binds to the end regions of growing microtubules. PMID: 22500803
    5. A mutation of the fission yeast EB1 overcomes negative regulation by phosphorylation and stabilizes microtubules. PMID: 22134091
    6. mal3 mutant phenotype can be suppressed by the presence of extra Spc7 PMID: 15371542
    7. These results show that microtubule dynamics is important for coordinated execution of mitotic events, in which Mal3 plays a vital role. PMID: 16394105
    8. Using electron microscopy (EM) and high-resolution surface shadowing we have studied the microtubule-binding properties of the fission yeast EB1 homolog Mal3p. PMID: 17190604
    9. Mal3 promotes assembly by binding to freshly formed tubulin polymer and particularly favors any with A-lattice arrangement PMID: 18794845
    10. Mal3 stabilizes microtubules not by preventing catastrophe at the microtubule tip but by inhibiting lattice depolymerization and enhancing rescue. PMID: 19740752

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton. Note=Associated with microtubules.
  • 蛋白家族：
    MAPRE family
  • 數據庫鏈接：


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