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  4. Recombinant Schizosaccharomyces pombe Mediator of replication checkpoint protein 1 (mrc1), partial

Recombinant Schizosaccharomyces pombe Mediator of replication checkpoint protein 1 (mrc1), partial

  • 中文名稱：
    龐貝裂殖酵母mrc1重組蛋白
  • 貨號：
    CSB-YP889248SXV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母mrc1重組蛋白
  • 貨號：
    CSB-EP889248SXV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母mrc1重組蛋白
  • 貨號：
    CSB-EP889248SXV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母mrc1重組蛋白
  • 貨號：
    CSB-BP889248SXV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母mrc1重組蛋白
  • 貨號：
    CSB-MP889248SXV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    mrc1; SPAC694.06c; Mediator of replication checkpoint protein 1; DNA replication checkpoint mediator mrc1
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Component of the replisome and is required for rad3-dependent activation of the checkpoint kinase cds1 in response to replication fork arrest. Phosphorylation allows it to mediate the activation of cds1.
  • 基因功能參考文獻(xiàn)：
    1. Results identify S. pombe Mrc1 with major role in mediating efficient replication stalling. Its function is independent of the protein's checkpoint activity, but dependent on a helix-turn-helix DNA-binding domain. PMID: 26201080
    2. Results show a novel checkpoint-independent function of Mrc1 which may define early-firing origins among the potential origins scattered on the chromosome. PMID: 21518960
    3. The N-terminal region of the S. pombe replication checkpoint protein Mrc1 has a helix-loop-helix motif that is predicted to bind to DNA. [mrc1] PMID: 15471884
    4. S. pombe Tel2 plays important roles in the Mrc1-mediated replication checkpoint as well as in the Cds1-independent regulation of genome integrity. PMID: 17189249
    5. These results suggest that interactions of the Swi1-Swi3 complex and Hsk1 kinase with Mrc1 may play a role in cellular responses to stalled replication forks in fission yeast PMID: 19422421

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  • 亞細(xì)胞定位：
    Nucleus. Note=Associated with chromatin.
  • 數(shù)據(jù)庫鏈接：


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