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  4. Recombinant Schizosaccharomyces pombe DNA repair protein rhp9 (rhp9), partial

Recombinant Schizosaccharomyces pombe DNA repair protein rhp9 (rhp9), partial

  • 中文名稱：
    Recombinant Schizosaccharomyces pombe DNA repair protein rhp9(rhp9) ,partial
  • 貨號：
    CSB-YP308549SXV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe DNA repair protein rhp9(rhp9) ,partial
  • 貨號：
    CSB-EP308549SXV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe DNA repair protein rhp9(rhp9) ,partial
  • 貨號：
    CSB-EP308549SXV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe DNA repair protein rhp9(rhp9) ,partial
  • 貨號：
    CSB-BP308549SXV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe DNA repair protein rhp9(rhp9) ,partial
  • 貨號：
    CSB-MP308549SXV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    crb2; rhp9; SPBC342.05; DNA repair protein crb2; Checkpoint mediator protein crb2; Cut5-repeat binding protein 2; RAD9 protein homolog
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Essential for cell cycle arrest at the G1 and G2 stages following DNA damage by X-, and UV-irradiation, or inactivation of DNA ligase. Plays a role in the response to DNA damage. Interaction with rad4 via its phosphorylation sites in the N-terminus couples the DNA checkpoint apparatus to chromatin via interaction of its C-terminal BRCT domains with epigenetic modifications on histones H4 and H2A, respectively, in the G1/S phase of the cell cycle, and facilitates recruitment of the checkpoint kinase chk1.
  • 基因功能參考文獻：
    1. Data show that polar interactions of Crb2 serine-548 and lysine-619 with the phosphate group of phospho-H2A (gamma-H2A) are critical for Crb2 IRIF formation and checkpoint function. PMID: 20679485
    2. Findings argue that binding of the Crb2 BRCT repeats to pH2AX is critical for checkpoint activity and provide new insight into the mechanisms of chromatin-mediated genome stability. PMID: 20679488
    3. When the Crb2 mutants experience damaged replication forks upon inhibition of the religation activity of topoisomerase I, the Chk1 DNA damage pathway induces sustained activation of the spindle checkpoint PMID: 16107732
    4. Full Crb2 activity requires phosphorylation of threonine-215 by Cdc2. PMID: 16314498
    5. Histone modifications cooperate in the same Crb2 recruitment pathway, which also requires the Tudor and BRCT motifs in Crb2. PMID: 16778077
    6. Structural and functional analysis of the crb2 domain reveals distinct roles in checkpoint signaling and DNA damage repair. PMID: 18676809

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  • 亞細胞定位：
    Nucleus.
  • 數(shù)據(jù)庫鏈接：


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