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Recombinant Schizosaccharomyces pombe DNA repair protein rhp51 (rhp51)

  • 中文名稱:
    龐貝裂殖酵母rhp51重組蛋白
  • 貨號:
    CSB-YP330610SXV
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    龐貝裂殖酵母rhp51重組蛋白
  • 貨號:
    CSB-EP330610SXV
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    龐貝裂殖酵母rhp51重組蛋白
  • 貨號:
    CSB-EP330610SXV-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    龐貝裂殖酵母rhp51重組蛋白
  • 貨號:
    CSB-BP330610SXV
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    龐貝裂殖酵母rhp51重組蛋白
  • 貨號:
    CSB-MP330610SXV
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    rhp51
  • Uniprot No.:
  • 別名:
    rhp51; rad51; SPAC644.14c; DNA repair protein rhp51; RAD51 homolog
  • 種屬:
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-365
  • 氨基酸序列
    MADTEVEMQV SAADTNNNEN GQAQSNYEYD VNVQDEEDEA AAGPMPLQML EGNGITASDI KKIHEAGYYT VESIAYTPKR QLLLIKGISE AKADKLLGEA SKLVPMGFTT ATEYHIRRSE LITITTGSKQ LDTLLQGGVE TGSITELFGE FRTGKSQICH TLAVTCQLPI DMGGGEGKCL YIDTEGTFRP VRLLAVADRY GLNGEEVLDN VAYARAYNAD HQLELLQQAA NMMSESRFSL LVVDSCTALY RTDFSGRGEL SARQMHLARF MRTLQRLADE FGIAVVITNQ VVAQVDGISF NPDPKKPIGG NILAHSSTTR LSLRKGRGEQ RICKIYDSPC LPESEAIFAI NSDGVGDPKE IIAPV
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Required both for recombination and for the repair of DNA damage caused by X-rays. Binds to single and double-stranded DNA, in the presence of magnesium, and exhibits DNA-dependent ATPase activity. Promotes DNA strand annealing and strand exchange via DNA recombinase activity and forms helical nucleoprotein filaments.
  • 基因功能參考文獻:
    1. Data show that Rad51-dependent homologous recombination (HR) predominates in centromeres. PMID: 28977643
    2. Rad51 and Rad54 are required for gene silencing in centromeres, suggesting that homologous recombination also plays a role in the structure and function of centromeres. PMID: 27697832
    3. spindle assembly checkpoint-dependent prometaphase arrest occurred more frequently in pot1Delta rqh1-hd double mutants than in rqh1-hd single mutants in Rad51-dependent recombination intermediates at telomeres PMID: 24469396
    4. Rad22-Mus81-dependent (Rhp51-independent) pathway is an important mechanism for the repair of DNA damage in fission yeast PMID: 15486206
    5. The recombinase Rad51 forms helical nucleoprotein filaments on single-stranded DNA. PMID: 15899844
    6. Rhp51 action is controlled through an interplay between Fbh1 and the mediator proteins.(Rhp51) PMID: 16135800
    7. Rad51 protein localizes to linear elements. PMID: 16532353
    8. genes rhp51+ and rhp55+ are involved not only in the repair of induced DNA double-strand breaks, but also in the mechanism of mating type switching in fission yeast. PMID: 16756067
    9. fission yeast (Rhp51) and human (hRad51) RecA homologues promote duplex-duplex DNA-strand exchange in vitro PMID: 18256600
    10. Rad22 (the S. pombe Rad52 homolog) and the Swi5-Sfr1 complex have distinct roles for mediating Rhp51 loading onto replication protein A-precoated ssDNA. PMID: 18416603
    11. These data suggest that Rad51 suppresses rearrangements of centromere repeats that result in isochromosome formation. PMID: 18923422

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  • 亞細胞定位:
    Nucleus.
  • 蛋白家族:
    RecA family, RAD51 subfamily
  • 數據庫鏈接:


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