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  4. Recombinant Schizosaccharomyces pombe DNA repair and recombination protein rad22 (rad22)

Recombinant Schizosaccharomyces pombe DNA repair and recombination protein rad22 (rad22)

  • 中文名稱：
    龐貝裂殖酵母rad22重組蛋白
  • 貨號：
    CSB-YP330608SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rad22重組蛋白
  • 貨號：
    CSB-EP330608SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rad22重組蛋白
  • 貨號：
    CSB-EP330608SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rad22重組蛋白
  • 貨號：
    CSB-BP330608SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rad22重組蛋白
  • 貨號：
    CSB-MP330608SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rad22
  • Uniprot No.：
  • 別名：
    rad22; SPAC30D11.10; DNA repair and recombination protein rad22
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-469
  • 氨基酸序列
    MSFEQKQHVA SEDQGHFNTA YSHEEFNFLQ SSLTRKLGPE YVSRRSGPGG FSVSYIESWK AIELANEIFG FNGWSSSIRS INVDFMDENK ENGRISLGLS VIVRVTIKDG AYHEDIGYGS IDNCRGKASA FEKCKKEGTT DALKRALRNF GNSLGNCMYD KYYLREVGKM KPPTYHFDSG DLFRKTDPAA RESFIKKQKT LNSTRTVNNQ PLVNKGEQLA PRRAAELNDE QTREIEMYAD EELDNIFVED DIIAHLAVAE DTAHPAANNH HSEKAGTQIN NKDKGSHNSA KPVQRSHTYP VAVPQNTSDS VGNAVTDTSP KTLFDPLKPN TGTPSPKFIS ARAAAAAEGV VSAPFTNNFN PRLDSPSIRK TSIIDHSKSL PVQRASVLPI IKQSSQTSPV SNNSMIRDSE SIINERKENI GLIGVKRSLH DSTTSHNKSD LMRTNSDPQS AMRSRENYDA TVDKKAKKG
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Active in the repair of DNA damage and in mating-type switching. Probably involved in the repair of DNA double-strands breaks. Has a role in promoting S phase completion.
  • 基因功能參考文獻：
    1. Rad52 protein function study by fluorescne tagging.Rad22 protein tagging,a S. pombe is a Rad52 homologue.Presence of tagged Rad22 protein was confirmed by Western-blot and fluorescence microscopy. PMID: 24340529
    2. Rad22 plays dual roles in regulating meiotic recombination: activating Rad51 and inhibiting Dmc1. PMID: 24186976
    3. Rad22 can promote strand invasion to form a D-loop that can be cleaved by Mus81 PMID: 15486206
    4. Gel-filtration experiments and electron microscopic analyses indicate self-association of Rad22A and Rad22B and the formation of multimeric structure. PMID: 17208257
    5. mediates the formation and branch migration of Holliday recombination intermediates. (review) PMID: 18788459

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    RAD52 family
  • 數據庫鏈接：


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