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  4. Recombinant Schizosaccharomyces pombe DNA repair and recombination protein pif1, mitochondrial (pif1)

Recombinant Schizosaccharomyces pombe DNA repair and recombination protein pif1, mitochondrial (pif1)

  • 中文名稱：
    龐貝裂殖酵母pfh1重組蛋白
  • 貨號：
    CSB-YP892570SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母pfh1重組蛋白
  • 貨號：
    CSB-EP892570SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母pfh1重組蛋白
  • 貨號：
    CSB-EP892570SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母pfh1重組蛋白
  • 貨號：
    CSB-BP892570SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母pfh1重組蛋白
  • 貨號：
    CSB-MP892570SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    pfh1
  • Uniprot No.：
  • 別名：
    pfh1; pif1; rph1; SPBC887.14c; ATP-dependent DNA helicase pfh1; EC 3.6.4.12; DNA repair and recombination helicase pfh1; PIF1 helicase homolog; RRM3/PIF1 homolog 1
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Involved in the maintenance of mitochondrial (mtDNA). Required for both repair of mitochondrial DNA and recognition of a recombinogenic signal characterized by a 26-bp palindromic at sequence in the ery region of mitochondrial DNA. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. Required for the completion of S-phase.
  • 基因功能參考文獻：
    1. The nuclear isoform of Pfh1 (nPfh1) unwound both types of structure, and although the G4-stabilizing compound Phen-DC3 significantly enhanced their stability, nPfh1 still resolved them efficiently PMID: 27185885
    2. In budding and fission yeasts, Pif1 family helicases impact both telomerase-mediated and semi-conservative replication of telomeric DNA as well as recombination-mediated telomere lengthening. (Review) PMID: 27233114
    3. Pfh1 is an accessory DNA helicase that interacts with the replisome and promotes replication and suppresses DNA damage at hard-to-replicate sites. PMID: 27611590
    4. Pfh1 binds telomeric DNA.Pfh1 facilitates telomere replication.Pfh1 is a positive regulator of telomere length. PMID: 25303777
    5. The proteome of cells devoid of Pfh1 were analyzed and determined that gene products up- and down-regulated upon iron depletion in wild-type cells are constitutively misregulated in this mutant. PMID: 23115244
    6. Pfh1 promotes DNA replication and separation of converged replication forks and suppresses DNA damage at hard-to-replicate sites. PMID: 22426534
    7. These data demonstrate that Pfh1p has essential roles in the replication of both nuclear and mitochondrial DNA. PMID: 18725402

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  • 亞細胞定位：
    [Isoform Nuclear]: Nucleus, nucleolus.; [Isoform Mitochondrial]: Mitochondrion.
  • 蛋白家族：
    Helicase family, PIF1 subfamily
  • 數據庫鏈接：


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