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  4. Recombinant Schizosaccharomyces pombe Cell division control protein 12 (cdc12), partial

Recombinant Schizosaccharomyces pombe Cell division control protein 12 (cdc12), partial

  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Cell division control protein 12(cdc12) ,partial
  • 貨號：
    CSB-YP605929SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Cell division control protein 12(cdc12) ,partial
  • 貨號：
    CSB-EP605929SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Cell division control protein 12(cdc12) ,partial
  • 貨號：
    CSB-EP605929SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Cell division control protein 12(cdc12) ,partial
  • 貨號：
    CSB-BP605929SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Cell division control protein 12(cdc12) ,partial
  • 貨號：
    CSB-MP605929SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    cdc12
  • Uniprot No.：
  • 別名：
    cdc12; SPAC1F5.04cCell division control protein 12
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Plays a role in the cell cycle. Involved in cytokinesis. Component of the cell division ring. In the absence of profilin, caps the barbed end of actin filaments, thus preventing subunit addition and dissociation. In the presence of profilin, nucleates actin filaments that grow rapidly from their barbed ends.
  • 基因功能參考文獻：
    1. Cdc12 mechanoregulation facilitates actomyosin ring assembly. PMID: 28951543
    2. this paper defines a short motif within the N terminus of Cdc12 that binds directly to the F-BAR domain of the scaffolding protein Cdc15. PMID: 25688133
    3. Cdc12 is an efficient nucleator (one filament per approximately 3 Cdc12 dimers) that processively elongates profilin-actin at a moderate rate of 10 subunits s-1 muM-1, but lacks filament-bundling activity. PMID: 21865598
    4. cdc12p contributes at least two separable activities: actin assembly and one or more additional functions in cytokinesis initiation PMID: 20226666
    5. A mutational analysis of cdc12p was used to identify regions of cdc12p responsible for ring assembly and localization. PMID: 18305104
    6. most likely hypotheses to explain the incompatibility of human profilin-I with Cdc12p are differences in interactions with the proline-rich sequences in the FH1 domain of Cdc12p and wider "wings" that interact with actin PMID: 19028693
    7. Cdc12p can recruit SpTm to actin filaments and SpTm has diverse effects on Cdc12p-mediated actin assembly. PMID: 19244341

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  • 亞細胞定位：
    Nucleus. Note=Nuclear, and septum.
  • 蛋白家族：
    Formin homology family, BNI1 subfamily
  • 數據庫鏈接：


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