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  4. Recombinant Schizosaccharomyces pombe Autophagy-related protein 3 (atg3)

Recombinant Schizosaccharomyces pombe Autophagy-related protein 3 (atg3)

  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Autophagy-related protein 3(atg3)
  • 貨號：
    CSB-YP528580SXV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Autophagy-related protein 3(atg3)
  • 貨號：
    CSB-EP528580SXV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Autophagy-related protein 3(atg3)
  • 貨號：
    CSB-EP528580SXV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Autophagy-related protein 3(atg3)
  • 貨號：
    CSB-BP528580SXV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Autophagy-related protein 3(atg3)
  • 貨號：
    CSB-MP528580SXV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    atg3; SPBC3B9.06cAutophagy-related protein 3; Autophagy-related E2-like conjugation enzyme atg3
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-275
  • 氨基酸序列
    MAQRLTSAFL NWREHITPAS KTSDFENTGM ISPEEFVLAG DYLVSKFPTW SWECGDRIRG FLPKDKQYLV TRHVFCVQRN INIGVNEEWV DIETDDTRNK DDDQDDDAIS SIHSDTSDIA SAERLKGQSK ELSDSGPLPL KDEEDDDQMV SPVIKEDEGR YYDLYIVYDK YYRTPRLFLR GWNAGGQLLT MKDIYEDVSG EHAGKTVTME PFPHYHSHNT MASVHPCKHA SVLLKLIKQH RERNDPIRVD QYMVLFLKFV STMLPYFEID YTIQA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of atg8. The atg12-atg5 conjugate plays a role of an E3 and promotes the transfer of atg8 from atg3 to phosphatidylethanolamine (PE). This step is required for the membrane association of atg8. The formation of the atg8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The atg8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy. Plays a role in meiosis and sporulation.
  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    ATG3 family
  • 數據庫鏈接：


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