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  4. Recombinant Sapporo virus Genome polyprotein (ORF1), partial

Recombinant Sapporo virus Genome polyprotein (ORF1), partial

  • 中文名稱：
    Recombinant Sapporo virus Genome polyprotein(ORF1) ,partial
  • 貨號：
    CSB-YP757844SFAW
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Sapporo virus Genome polyprotein(ORF1) ,partial
  • 貨號：
    CSB-EP757844SFAW
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Sapporo virus Genome polyprotein(ORF1) ,partial
  • 貨號：
    CSB-EP757844SFAW-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Sapporo virus Genome polyprotein(ORF1) ,partial
  • 貨號：
    CSB-BP757844SFAW
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Sapporo virus Genome polyprotein(ORF1) ,partial
  • 貨號：
    CSB-MP757844SFAW
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ORF1
  • Uniprot No.：
  • 別名：
    ORF1; Genome polyprotein [Cleaved into: Protein p11; Protein p28; NTPase; EC 3.6.1.15; p35); Protein p32; Viral genome-linked protein; VPg; p14); Protease-polymerase p70; Pro-Pol; EC 2.7.7.48; EC 3.4.22.66); Capsid protein; CP; VP1; p60)]
  • 種屬：
    Sapporo virus (isolate GII/Human/Thailand/Mc10/2000) (Hu/SaV/Mc10/2000/Thailand)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity.; Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation.; Protease-polymerase p70 processes the polyprotein: Pro-Pol is first released by autocleavage, then all other proteins are cleaved. It is also an RNA-directed RNA polymerase which replicates genomic and antigenomic viral RNA by recognizing specific signals. Catalyzes the covalent attachment VPg with viral RNAs.; Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm.
  • 基因功能參考文獻：
    1. This study suggested the recombination site was at the polymerase-capsid junction within open reading frame one PMID: 15504283
    2. ORF1 polyprotein is processed into at least 10 major proteins: p11, p28, p35, p32, p14, p70, p60, p66, p46, and p120 by 3C-like protease PMID: 15919882
    3. VP1 cleaved from a part of ORF1 polyprotein self-assembled into virus-like particles in COS-7 cell. PMID: 19161558
  • 亞細胞定位：
    [Capsid protein]: Virion. Host cytoplasm.
  • 數據庫鏈接：

    KEGG: vg:2943313



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