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  4. Recombinant Salmonella typhimurium DNA-binding protein H-NS (hns)

Recombinant Salmonella typhimurium DNA-binding protein H-NS (hns)

  • 中文名稱：
    鼠傷寒沙門菌hns重組蛋白
  • 貨號：
    CSB-YP358108SXB
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌hns重組蛋白
  • 貨號：
    CSB-EP358108SXB
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌hns重組蛋白
  • 貨號：
    CSB-EP358108SXB-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌hns重組蛋白
  • 貨號：
    CSB-BP358108SXB
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌hns重組蛋白
  • 貨號：
    CSB-MP358108SXB
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    hns
  • Uniprot No.：
  • 別名：
    hns; hnsA; osmZ; STM1751DNA-binding protein H-NS; Histone-like protein HLP-II; Protein B1; Protein H1
  • 種屬：
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-137
  • 氨基酸序列
    SEALKILNN IRTLRAQARE CTLETLEEML EKLEVVVNER REEESAAAAE VEERTRKLQQ YREMLIADGI DPNELLNSMA AAKSGTKAKR AARPAKYSYV DENGETKTWT GQGRTPAVIK KAMEEQGKQL EDFLIKE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Binds tightly to dsDNA. Acts as a global transcriptional regulator through its ability to bind to AT-rich DNA sequences. Binds in the minor groove of AT-rich DNA. Was found to bind 746 genes, about half of which show no change in expression in disruption experiments suggesting these sites are important for nucleoid structure. On a global level genes bound by H-NS are expressed at a lower than average level; H-NS is excluded from binding to highly transcribed genes and does not co-localize with RNA polymerase in DNA-binding studies during exponential growth in rich medium. The best correlation for H-NS binding is AT-content rather than predicted DNA curvature. Has a strong preference for DNA that has been recently acquired by horizontal gene transfer, binding strongly to Salmonella pathogenicity islands 1 and 2 (SPI1 and SPI2); this offers the selective advantage of silencing foreign DNA while keeping it in the genome in case of need. DNA-binding influences plasmid DNA topology. For the proU locus (the proV-proW-proX operon) inhibits transcription at low osmolarity by binding to AT-rich, curved, DNA downstream of the transcription start site of proV; repression is greater when more than 1 curved DNA sequence is present. It plays a role in the thermal control of pili production. It is subject to transcriptional auto-repression. It binds preferentially to the upstream region of its own gene recognizing two segments of DNA on both sides of a bend centered around -150.
  • 基因功能參考文獻：
    1. Data suggest that Sfh acts as a molecular backup for H-NS and that it provides its 'stealth' function by replacing H-NS on the chromosome, thus minimizing disturbances to the H-NS-DNA binding pattern in cells that acquire pSfR27. PMID: 20444106
    2. The effects of heat on the oligomerization and gene regulating activity of HNS in S. typhimurium are reported. PMID: 15966862
    3. H-NS selectively silences horizontally acquired genes by targeting sequences with GC content lower than the resident genome; H-NS provides a previously unrecognized mechanism of bacterial defense against foreign DNA PMID: 16763111
    4. HilA promotes SPI4 expression by antagonizing the global transcriptional silencer H-NS. PMID: 18160484
    5. H-NS-like proteins have evolved to selectively target horizontally acquired and not core genome DNA. PMID: 19521501

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  • 亞細胞定位：
    Cytoplasm, nucleoid.
  • 蛋白家族：
    Histone-like protein H-NS family
  • 數據庫鏈接：

    KEGG: stm:STM1751

    STRING: 99287.STM1751



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