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  4. Recombinant Saccharomyces cerevisiae tRNA (guanine (9)-N1)-methyltransferase (TRM10)

Recombinant Saccharomyces cerevisiae tRNA (guanine (9)-N1)-methyltransferase (TRM10)

  • 中文名稱：
    釀酒酵母TRM10重組蛋白
  • 貨號：
    CSB-YP615502SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母TRM10重組蛋白
  • 貨號：
    CSB-EP615502SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母TRM10重組蛋白
  • 貨號：
    CSB-EP615502SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母TRM10重組蛋白
  • 貨號：
    CSB-BP615502SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母TRM10重組蛋白
  • 貨號：
    CSB-MP615502SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TRM10
  • Uniprot No.：
  • 別名：
    TRM10; YOL093W; O0926; tRNA; guanine(9)-N1)-methyltransferase; EC 2.1.1.221; tRNA methyltransferase 10; tRNA(m1G9)-methyltransferase; tRNA(m1G9)MTase
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-293
  • 氨基酸序列
    MSNDEINQNE EKVKRTPPLP PVPEGMSKKQ WKKMCKRQRW EENKAKYNAE RRVKKKRLRH ERSAKIQEYI DRGEEVPQEL IREPRINVNQ TDSGIEIILD CSFDELMNDK EIVSLSNQVT RAYSANRRAN HFAEIKVAPF DKRLKQRFET TLKNTNYENW NHFKFLPDDK IMFGDEHISK DKIVYLTADT EEKLEKLEPG MRYIVGGIVD KNRYKELCLK KAQKMGIPTR RLPIDEYINL EGRRVLTTTH VVQLMLKYFD DHNWKNAFES VLPPRKLDAE AKSASSSPAP KDT
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    S-adenosyl-L-methionine-dependent guanine N(1)-methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in cytoplasmic tRNAs.
  • 基因功能參考文獻：
    1. Mechanistic features of the atypical tRNA m1G9 SPOUT methyltransferase, Trm10, has been reported. PMID: 28911116
    2. The findings revealed that Trm10 is a new member of the SpoU-TrmD (SPOUT) MTase superfamily. PMID: 24081582
    3. The in vitro methylation activity of Trm10 is not sufficient to explain the observed pattern of modification in vivo, as additional tRNA species are substrates for Trm10 m1G9 methyltransferase activity. PMID: 23793893
    4. Our results also provide genetic evidence for a new role of Trm10p in translation termination efficiency. PMID: 21829385
  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Class IV-like SAM-binding methyltransferase superfamily, TRM10 family
  • 數據庫鏈接：

    KEGG: sce:YOL093W

    STRING: 4932.YOL093W



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