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Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein IST1 (IST1)

  • 中文名稱:
    釀酒酵母IST1重組蛋白
  • 貨號:
    CSB-YP345709SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母IST1重組蛋白
  • 貨號:
    CSB-EP345709SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母IST1重組蛋白
  • 貨號:
    CSB-EP345709SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母IST1重組蛋白
  • 貨號:
    CSB-BP345709SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母IST1重組蛋白
  • 貨號:
    CSB-MP345709SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    IST1; YNL265C; N0809; Vacuolar protein sorting-associated protein IST1; Increased sodium tolerance protein 1
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-298
  • 氨基酸序列
    MAPSMIPFTI KLKTCLKMCI QRLRYAQEKQ QAIAKQSRRQ VAQLLLTNKE QKAHYRVETL IHDDIHIELL EILELYCELL LARVQVINDI STEEQLVKEH MDDGINEAIR SLIYAILFVD EVKELSQLKD LMAWKINVEF VNGVIADHID VPEKIIKKCS PSVPKEELVD LYLKEIAKTY DVPYSKLENS LSSSSSNISS DFSDPSGDIE DNDEEKPILA LDNDDNDNAD AKHPITVKKP RQNSENIKNE LKIPKDIKKE VIEKKQSEKK TTKRKTKKEQ ENDELDELKK RFDALRRK
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Involved in a late step in sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins are targeted into the vacuole after fusion of the endosome with the vacuole. Regulates the recruitment of VPS4 to the ESCRT-III complex, probably in conjunction with DID2, and VPS4 catalyzes the disassembly of the ESCRT-III complex.
  • 基因功能參考文獻:
    1. data support a model in which Ist1-Did2 interactions during ESCRT-III polymerization coordinate Vps4 activity with the timing of ESCRT-III disassembly PMID: 26515066
    2. Ist1 has a dual role in the regulation of Vps4 activity. PMID: 18032582
  • 亞細胞定位:
    Cytoplasm. Endosome. Note=The endosomal location appears to be dependent on DID2.
  • 蛋白家族:
    IST1 family
  • 數據庫鏈接:

    KEGG: sce:YNL265C

    STRING: 4932.YNL265C



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