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  4. Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 21 (VPS21)

Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 21 (VPS21)

  • 中文名稱：
    釀酒酵母VPS21重組蛋白
  • 貨號：
    CSB-YP334064SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母VPS21重組蛋白
  • 貨號：
    CSB-EP334064SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母VPS21重組蛋白
  • 貨號：
    CSB-EP334064SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母VPS21重組蛋白
  • 貨號：
    CSB-BP334064SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母VPS21重組蛋白
  • 貨號：
    CSB-MP334064SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    VPS21
  • Uniprot No.：
  • 別名：
    VPS21; VPS12; VPT12; YPT21; YPT51; YOR089C; YOR3154C; Vacuolar protein sorting-associated protein 21; GTP-binding protein YPT51; Vacuolar protein-targeting protein 12
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-210
  • 氨基酸序列
    MNTSVTSIKL VLLGEAAVGK SSIVLRFVSN DFAENKEPTI GAAFLTQRVT INEHTVKFEI WDTAGQERFA SLAPMYYRNA QAALVVYDVT KPQSFIKARH WVKELHEQAS KDIIIALVGN KIDMLQEGGE RKVAREEGEK LAEEKGLLFF ETSAKTGENV NDVFLGIGEK IPLKTAEEQN SASNERESNN QRVDLNAAND GTSANSACSC
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for protein transport to the vacuole. Involved in two vesicle trafficking steps to the prevacuolar compartment (PVC), regulating the docking of endosomes and Golgi vesicles to the PVC by interacting with PEP7/VAC1 on the PVC membrane and promoting SNARE complex formation.
  • 基因功能參考文獻：
    1. Data indicate that efficient Vps21 recycling requires both Ypt7 and endosome-vacuole fusion, thus suggesting extended control of a GAP cascade beyond Rab interactions. PMID: 25971802
    2. Endosomal class E compartments accumulate chronically active Vps21, the yeast Rab5A ortholog. PMID: 22899724
    3. CORVET (class C core vacuole/endosome tethering) binds to SNAREs and Rab5/Vps21-GTP and can specifically tether Vps21-positive membranes. PMID: 23417307
    4. Gyp3 spatially restricts active Vps21 to intermediate endosomes. PMID: 22748138
    5. Msb3 forms a specific transition complex with Vps21 and has the highest activity of all recombinant GAPs for Vps21 PMID: 22593206
    6. regulation of PI(3)P synthesis by Rab5 and Vps21 is essential for TORC1 function in both contexts. PMID: 22547071
    7. Data show that that Vps21, a Saccharomyces cerevisiae ortholog of mammalian endosomal Rab5, functions in trans with itself and with at least two other endosomal Rabs to directly mediate GTP-dependent tethering. PMID: 22157956
    8. Vps8 interacts and cooperates with the activated Rab5 homolog Vps21 to induce the clustering of late endosomal membranes. PMID: 19828734
    9. Transport among Golgi, endosome, and vacuole is controlled by two homologous tethering complexes, the previously identified HOPS complex at the vacuole and a novel endosomal tethering (CORVET) complex, which interacts with the Rab GTPase Vps21. PMID: 17488625

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  • 亞細胞定位：
    Endosome membrane; Lipid-anchor. Mitochondrion membrane; Lipid-anchor.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 數據庫鏈接：

    KEGG: sce:YOR089C

    STRING: 4932.YOR089C



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