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  4. Recombinant Saccharomyces cerevisiae Ubiquitin carboxyl-terminal hydrolase 10 (UBP10), partial

Recombinant Saccharomyces cerevisiae Ubiquitin carboxyl-terminal hydrolase 10 (UBP10), partial

  • 中文名稱：
    釀酒酵母UBP10重組蛋白
  • 貨號：
    CSB-YP025696SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母UBP10重組蛋白
  • 貨號：
    CSB-EP025696SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母UBP10重組蛋白
  • 貨號：
    CSB-EP025696SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母UBP10重組蛋白
  • 貨號：
    CSB-BP025696SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母UBP10重組蛋白
  • 貨號：
    CSB-MP025696SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    UBP10
  • Uniprot No.：
  • 別名：
    UBP10; DOT4; YNL186W; N1619; Ubiquitin carboxyl-terminal hydrolase 10; EC 3.4.19.12; Deubiquitinating enzyme 10; Disrupter of telomere silencing protein 4; Ubiquitin thioesterase 10; Ubiquitin-specific-processing protease 10
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Deubiquitinating enzyme involved in telomere and HM loci silencing, which is the repression of chromatin structure which leads to a stop in the transcription of nearby genes. Targets histone H2B for deubiquitination, thus helping to localize SIR2 to the telomere. At silent chromatin, including telomeres and the rDNA locus, not only maintains low H2B 'Lys-123' ubiquitination (H2BK123Ub), but also low H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). Controls the proliferating-cell nuclear antigen PCNA/POL30 deubiquitination which is crucial for keeping TLS polymerases in check as well as for down-regulating the error-free bypass. Deubiquitinates and stabilizes RPA190, the largest subunit of RNA polymerase I, to achieve optimal levels of ribosomes and cell growth. Protects also nutrient transporters such as GAP1 from ubiquitin-dependent endocytosis.
  • 基因功能參考文獻：
    1. show that Ubp10 possesses multiple, distinct binding modules within its IDRs that are necessary and sufficient for directing protein interactions important for Ubp10's known roles in gene silencing and ribosome biogenesis PMID: 26149687
    2. In this work we present clear evidence indicating that Ubp10 controls PCNA deubiquitylation in S. cerevisiae PMID: 22829782
  • 亞細胞定位：
    Nucleus. Chromosome, telomere. Nucleus, nucleolus.
  • 蛋白家族：
    Peptidase C19 family
  • 數據庫鏈接：

    KEGG: sce:YNL186W

    STRING: 4932.YNL186W



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