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  4. Recombinant Saccharomyces cerevisiae Transmembrane GTPase FZO1 (FZO1), partial

Recombinant Saccharomyces cerevisiae Transmembrane GTPase FZO1 (FZO1), partial

  • 中文名稱：
    釀酒酵母FZO1重組蛋白
  • 貨號：
    CSB-YP339948SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母FZO1重組蛋白
  • 貨號：
    CSB-EP339948SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母FZO1重組蛋白
  • 貨號：
    CSB-EP339948SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母FZO1重組蛋白
  • 貨號：
    CSB-BP339948SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母FZO1重組蛋白
  • 貨號：
    CSB-MP339948SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    FZO1
  • Uniprot No.：
  • 別名：
    FZO1; YBR179C; YBR1241Mitofusin FZO1; EC 3.6.5.-; Transmembrane GTPase FZO1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion proceeds through several steps; first mitochondria are tethered together, then brought into close contact, followed by the formation of a docking ring around contact areas, and finally membrane fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondrial morphology, which is balanced between fusion and fission, mediated by FZO1 and DNM1, respectively. Functions antagonistically with DNM1. Probably acts by forming membrane contact sites that mediate mitochondrial membrane fusion. Mitochondrial docking and fusion requires GTP hydrolysis. Mitochondrial fusion promotes also increased lifespan.
  • 基因功能參考文獻：
    1. The Saccharomyces cerevisiae Fzo1 and animal Mitofusin proteins are highly diverged from one another and lack strong sequence similarity. PMID: 29212658
    2. The results indicated that two different Ubp3 and Ubx2 complexes independently regulate mitochondrial fusion and Fzo1 turnover. PMID: 29924334
    3. Results identify diverse effects of CMT2A mutations, including a possible role for Mfn2 ubiquitylation and degradation in CMT2A pathogenesis, and provide evidence for a link between Fzo1 GTP hydrolysis, ubiquitylation, and mitochondrial fusion. PMID: 19812251
    4. homotypic trans interactions of Fzo1 were required to promote the fusion of mitochondrial outer membranes PMID: 15297626
    5. proteasomal degradation of Fzo1 in response to the mating pheromone may have a role in mediating the remodeling of the mitochondrial network during the process of mating PMID: 15760898
    6. Fzo1 oligomer domain interactions are essential for mitochondrial fusion PMID: 16624808
    7. This study provides a framework for developing an understanding of the function of Mdm30p-mediated Fzo1p degradation in the multistep process of mitochondrial fusion. PMID: 18353967

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  • 亞細胞定位：
    Mitochondrion outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family, Mitofusin subfamily
  • 數據庫鏈接：

    KEGG: sce:YBR179C

    STRING: 4932.YBR179C



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