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  4. Recombinant Saccharomyces cerevisiae Translation initiation factor eIF-2B subunit beta (GCD7)

Recombinant Saccharomyces cerevisiae Translation initiation factor eIF-2B subunit beta (GCD7)

  • 中文名稱：
    釀酒酵母GCD7重組蛋白
  • 貨號：
    CSB-YP007515SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母GCD7重組蛋白
  • 貨號：
    CSB-EP007515SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母GCD7重組蛋白
  • 貨號：
    CSB-EP007515SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母GCD7重組蛋白
  • 貨號：
    CSB-BP007515SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母GCD7重組蛋白
  • 貨號：
    CSB-MP007515SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GCD7
  • Uniprot No.：
  • 別名：
    GCD7; TIF222; YLR291C; L8003.17; Translation initiation factor eIF-2B subunit beta; GCD complex subunit GCD7; Guanine nucleotide exchange factor subunit GCD7; eIF-2B GDP-GTP exchange factor subunit beta
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-381
  • 氨基酸序列
    MSSQAFTSVH PNAATSDVNV TIDTFVAKLK RRQVQGSYAI ALETLQLLMR FISAARWNHV NDLIEQIRDL GNSLEKAHPT AFSCGNVIRR ILAVLRDEVE EDTMSTTVTS TSVAEPLISS MFNLLQKPEQ PHQNRKNSSG SSSMKTKTDY RQVAIQGIKD LIDEIKNIDE GIQQIAIDLI HDHEILLTPT PDSKTVLKFL ITARERSNRT FTVLVTEGFP NNTKNAHEFA KKLAQHNIET LVVPDSAVFA LMSRVGKVII GTKAVFVNGG TISSNSGVSS VCECAREFRT PVFAVAGLYK LSPLYPFDVE KFVEFGGSQR ILPRMDPRKR LDTVNQITDY VPPENIDIYI TNVGGFNPSF IYRIAWDNYK QIDVHLDKNK A
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Acts as a regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD6 and GCD7 repress GCN4 expression at the translational level by ensuring that ribosomes which have translated UORF1 will reinitiate at UORF2, -3, or -4 and thus fail to reach the GCN4 start site.
  • 基因功能參考文獻：
    1. findings provide compelling evidence that beta/Gcd7 is crucial for binding of substrate by eIF2B in vivo, beyond its dispensable regulatory role in the inhibition of eIF2B by eIF PMID: 20805354
    2. The eIF2/eIF5 complex represents a cytoplasmic reservoir for eIF2 that antagonizes eIF2B-promoted guanine nucleotide exchange, enabling coordinated regulation of translation initiation. PMID: 16990799
    3. These data show that multiple contacts between eIF2gamma and eIF2Bepsilon mediate nucleotide exchange. PMID: 17526738
  • 蛋白家族：
    EIF-2B alpha/beta/delta subunits family
  • 數據庫鏈接：

    KEGG: sce:YLR291C

    STRING: 4932.YLR291C



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