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  4. Recombinant Saccharomyces cerevisiae Transcription initiation factor IIF subunit alpha (TFG1), partial

Recombinant Saccharomyces cerevisiae Transcription initiation factor IIF subunit alpha (TFG1), partial

  • 中文名稱：
    釀酒酵母TFG1重組蛋白
  • 貨號：
    CSB-YP336930SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母TFG1重組蛋白
  • 貨號：
    CSB-EP336930SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母TFG1重組蛋白
  • 貨號：
    CSB-EP336930SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母TFG1重組蛋白
  • 貨號：
    CSB-BP336930SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母TFG1重組蛋白
  • 貨號：
    CSB-MP336930SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TFG1
  • Uniprot No.：
  • 別名：
    TFG1; SSU71; YGR186W; G7526; Transcription initiation factor IIF subunit alpha; TFIIF-alpha; TFIIF large subunit; Transcription factor G 105 kDa subunit; P105
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation.
  • 基因功能參考文獻：
    1. Tfg1 C-terminal domain interacts with double-stranded DNA oligonucleotides. PMID: 22095626
    2. Monitoring elongation complexes containing RNAPII and combinations of TFIIF (SSU71) and TFIIS to mechanical loads, found that both factors are independently capable of restoring arrested RNAPII to productive elongation. PMID: 24733897
    3. Evidence that the Tfg1/Tfg2 dimer interface of TFIIF is located in proximity to the B-finger of RNA polymerase II (TFIIB) near the active center of RNAP II where the TFIIB-TFIIF-RNAP II interface plays a key role in transcription start site selection. PMID: 16147988
    4. TFIIF stimulates formation of the first two phosphodiester bonds and dramatically stabilizes a short RNA-DNA hybrid in the RNA polymerase II (RNAPII) active center. PMID: 18362165
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    TFIIF alpha subunit family
  • 數據庫鏈接：

    KEGG: sce:YGR186W

    STRING: 4932.YGR186W



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